Analysis of pYC2, a cryptic plasmid in Lactobacillus sakei BM5 isolated from kimchi

Analysis of the structural properties of pYC2, a cryptic plasmid from Lactobacillus sakei BM5 isolated from kimchi, determined its length as 1,970 bp with a G + C content of 34%. The double-strand origin (dso) and single-strand origin (sso) of rolling-circle replicating plasmids were found in the nu...

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Bibliographic Details
Published in:Biotechnology letters 2009, Vol.31 (1), p.123-130
Main Authors: Yang, Eun Ju, Chang, Hae Choon
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Applied Microbiology
Bacteria
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Base Sequence
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Blotting, Southern
Cloning, Molecular
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Electrophoresis, Agar Gel
Food Microbiology
Fundamental and applied biological sciences. Psychology
Genetically altered foods
Homology
Hybridization
Kimchi
Lactobacillus - genetics
Lactobacillus - isolation & purification
Lactobacillus sakei
Life Sciences
Microbiology
Molecular Sequence Data
Nucleotides
Open Reading Frames - genetics
Original Research Paper
Origins
Phylogeny
Plasmids - analysis
Plasmids - genetics
Plasmids - isolation & purification
Proteins
Replicating
Sequence Analysis, DNA
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Summary:Analysis of the structural properties of pYC2, a cryptic plasmid from Lactobacillus sakei BM5 isolated from kimchi, determined its length as 1,970 bp with a G + C content of 34%. The double-strand origin (dso) and single-strand origin (sso) of rolling-circle replicating plasmids were found in the nucleotide sequence of the pYC2 plasmid. Sequence analysis of pYC2 revealed that ORF 1 and ORF 2 showed high homology with the Cop and Rep proteins encoded by the pMV158 family of plasmids replicating via the rolling-circle mechanism. pYC2 also replicates by this mechanism, as confirmed by Southern hybridization analysis.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-008-9842-y