An Origin of DNA Replication and a Transcription Silencer Require a Common Element

A eukaryotic chromosomal origin of replication was identified in the yeast Saccharomyces cerevisiae. By several criteria, including map position, deletion analysis, and a synthetic form of saturation mutagenesis, the origin co-localized with the HMR-E silencer, which is a DNA element that represses...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1992-05, Vol.256 (5057), p.659-663
Main Authors: Rivier, David H., Rine, Jasper
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell cycle
Cellular biology
Chromosome Mapping
Consensus sequence
Deoxyribonucleic acid
DNA
DNA Replication
DNA, Fungal - biosynthesis
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Fungal
Genes
Genes, Fungal
Genetic loci
Genetic regulation
Genetic transcription
Molecular and cellular biology
Molecular genetics
Molecules
Mutagenesis
Nucleic Acid Hybridization
Regulation
Regulatory Sequences, Nucleic Acid
Replication
Replication origin
Repression
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Silencers
Transcription (Genetics)
Transcription, Genetic
Yeasts
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Summary:A eukaryotic chromosomal origin of replication was identified in the yeast Saccharomyces cerevisiae. By several criteria, including map position, deletion analysis, and a synthetic form of saturation mutagenesis, the origin co-localized with the HMR-E silencer, which is a DNA element that represses transcription of the adjacent genes. A specific site within the silencer was required for both initiation of chromosomal replication and for repression of transcription. This analysis directly demonstrates that initiation of eukaryotic chromosomal replication is dependent on specific sequence elements and that a particular element can act in both initiation of chromosomal replication and regulation of transcription.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1585179