Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis

Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-d...

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Bibliographic Details
Published in:Biotechnology letters 2006-11, Vol.28 (22), p.1805-1810
Main Authors: Jun, Hee Jin, Chung, Mi Ja, Kim, Sang Yeon, Lee, Ho Joung, Lee, Sung Joon
Format: Article
Language:English
Subjects:
Adhesion
alpha-Tocopherol - chemistry
Anti-atherogenic
Atherosclerosis
Biological and medical sciences
Biotechnology
Cell Adhesion
Cells
Cells, Cultured
Coculture Techniques
Colorimetry - instrumentation
Colorimetry - methods
Computational Biology - instrumentation
Computational Biology - methods
Dose-Response Relationship, Drug
Endothelial Cells - cytology
Endothelium/monocyte binding
Fundamental and applied biological sciences. Psychology
gamma-Tocopherol - chemistry
Humans
Lipopolysaccharides - metabolism
Lithospermum - metabolism
Lithospermum erythrorhizon
Monocytes - cytology
Monocytes - metabolism
Plant Roots
Citations: Items that this one cites
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Summary:Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-dependent manner and the adhesion was attenuated with post-treatments with l-ascorbic acid (53%), α- (40%) and γ-tocopherol (39%), resveratrol (39%), and Lithospermum erythrorhizon root extract (45%). This non-radioactive, colorimetric assay may be useful for screening anti-atherogenic compounds in early atherogenesis.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-006-9165-9