Phosphorylation-regulated CI − channel in CHO cells stably expressing the cystic fibrosis gene

A cyclic AMP-stimulated chloride conductance appears when the cystic fibrosis gene is expressed in non-epithelial cells by infection with recombinant viruses. Cyclic AMP-stimulated conductance in this system is mediated by the same ohmic, low-conductance Cl- channel as in human secretory epithelia,...

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Bibliographic Details
Published in:Nature (London) 1991-08, Vol.352 (6336), p.628-631
Main Authors: Tabcharani, Joseph A, Chang, Xiu-Bao, Riordan, John R, Hanrahan, John W
Format: Article
Language:English
Subjects:
Biochemistry
Cystic fibrosis
Genetics
Medical research
Rodents
Synergism
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Summary:A cyclic AMP-stimulated chloride conductance appears when the cystic fibrosis gene is expressed in non-epithelial cells by infection with recombinant viruses. Cyclic AMP-stimulated conductance in this system is mediated by the same ohmic, low-conductance Cl- channel as in human secretory epithelia, but control of this channel by phosphorylation has not been directly demonstrated. Here we report the appearance of the low-conductance Cl- channel in Chinese hamster ovary cells after stable transfection with the cystic fibrosis gene. The channel is regulated on-cell by membrane-permeant analogues of cAMP and off-cell by protein kinases A and C and by alkaline phosphatase. These results are further evidence that the cystic fibrosis transmembrane regulator is a Cl- channel which can be activated by specific phosphorylation events and inactivated by dephosphorylation; they reveal an unsuspected synergism between converging kinase regulatory pathways.
ISSN:0028-0836
1476-4687
DOI:10.1038/352628a0