Loading…
Plasma sampling and freezing procedures influence vitellogenin measurements by enzyme-linked immunoassay in the fathead minnow (Pimephales promelas)
The present study compared three different methods for measuring plasma vitellogenin (VTG) in fathead minnow (FHM; Pimephales promelas): A procedure using liquid chromatography with electrospray ionization combined with tandem mass spec‐trometry (LC/ESI‐MS/MS), and two commercial enzyme‐linked immun...
Saved in:
Published in: | Environmental toxicology and chemistry 2006-02, Vol.25 (2), p.337-348 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c5175-219ba94793758ddf2c6949e60f56520f6d6fe3df2d864f1d9f99a1af1342d9913 |
---|---|
cites | cdi_FETCH-LOGICAL-c5175-219ba94793758ddf2c6949e60f56520f6d6fe3df2d864f1d9f99a1af1342d9913 |
container_end_page | 348 |
container_issue | 2 |
container_start_page | 337 |
container_title | Environmental toxicology and chemistry |
container_volume | 25 |
creator | Brodeur, Julie C. Woodburn, Kent B. Zhang, Fagen Bartels, Michael J. Klecka, Gary M. |
description | The present study compared three different methods for measuring plasma vitellogenin (VTG) in fathead minnow (FHM; Pimephales promelas): A procedure using liquid chromatography with electrospray ionization combined with tandem mass spec‐trometry (LC/ESI‐MS/MS), and two commercial enzyme‐linked immunoassay (ELISA) kits using either anti‐carp or anti‐FHM antibodies. The influence on plasma VTG measurements of using the protease‐inhibitor aprotinin during blood sampling and of submitting the plasma samples to a freeze–thaw cycle before analysis also was evaluated. The addition of aprotinin to the blood during sampling significantly reduced the plasma VTG concentrations measured by ELISA, whereas the VTG values measured after plasma samples were submitted to a freeze–thaw cycle were significantly higher than those measured before freezing. This inflating effect of freezing on VTG measurements made by ELISA could be prevented if plasma samples were frozen diluted in citrate buffer containing 16 mg/ml of polyethylene glycol (PEG). In contrast, measurements of VTG made by LC/ESI‐MS/MS were unaffected by freezing and, conceptually, are independent from enzymatic degradation. Although the use of aprotinin and PEG effectively reduced the influence of enzymatic and physical degradation caused by freezing and thawing on VTG measurements made by ELISA, it did not improve agreement between the three analytical techniques evaluated. More information is needed regarding the molecular structure and the existence of possible multiple forms of VTG before this protein can be measured adequately in FHM. |
doi_str_mv | 10.1897/05-368R.1 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_743379571</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67721061</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5175-219ba94793758ddf2c6949e60f56520f6d6fe3df2d864f1d9f99a1af1342d9913</originalsourceid><addsrcrecordid>eNqNkstu1TAQhiMEoqeFBS-ALCQuXaT4EtvxElVtQZRSEJSl5ROPW7exc4gTSvocPDCOzhGVkLhsbHn8zf-Px1MUjwjeI7WSLzEvmag_7pE7xYJwTstakPpuscCS4VJSUW8V2yldYkyEUup-sUUEJ4oqtih-nLYmBYOSCavWx3NkokWuB7iZD6u-a8COPSTko2tHiA2gb36Atu3OIfqIApiU7wPEIaHlhCDeTAHKLHUFFvkQxtiZlMyUBdBwAciZvBqLgo-xu0YvTn2A1YVps0V2C5DL2X1Q3HOmTfBws-8Unw8PPu2_Lo_fH73Zf3VcNpxIXlKilkZVUjHJa2sdbYSqFAjsuOAUO2GFA5bjthaVI1Y5pQwxjrCKWqUI2ymer3Wz89cR0qCDT01-nInQjUnLijGpuJzJZ38lhZSUYPFvkBKWi6n_B8SVrInI4JPfwMtu7GPuy8wwzjDhGdpdQ03fpdSD06veB9NPmmA9z4jGXM8zomfnxxvBcRnA3pKbocjA0w1gUmNa15vY-HTLzW1hVZ25as1d-xamPzvqDHFBMeWY4rnWcp3m0wDff6WZ_iq3MX-l_nJypA_fneGztycftGQ_Aaib44U</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>210353015</pqid></control><display><type>article</type><title>Plasma sampling and freezing procedures influence vitellogenin measurements by enzyme-linked immunoassay in the fathead minnow (Pimephales promelas)</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Brodeur, Julie C. ; Woodburn, Kent B. ; Zhang, Fagen ; Bartels, Michael J. ; Klecka, Gary M.</creator><creatorcontrib>Brodeur, Julie C. ; Woodburn, Kent B. ; Zhang, Fagen ; Bartels, Michael J. ; Klecka, Gary M.</creatorcontrib><description>The present study compared three different methods for measuring plasma vitellogenin (VTG) in fathead minnow (FHM; Pimephales promelas): A procedure using liquid chromatography with electrospray ionization combined with tandem mass spec‐trometry (LC/ESI‐MS/MS), and two commercial enzyme‐linked immunoassay (ELISA) kits using either anti‐carp or anti‐FHM antibodies. The influence on plasma VTG measurements of using the protease‐inhibitor aprotinin during blood sampling and of submitting the plasma samples to a freeze–thaw cycle before analysis also was evaluated. The addition of aprotinin to the blood during sampling significantly reduced the plasma VTG concentrations measured by ELISA, whereas the VTG values measured after plasma samples were submitted to a freeze–thaw cycle were significantly higher than those measured before freezing. This inflating effect of freezing on VTG measurements made by ELISA could be prevented if plasma samples were frozen diluted in citrate buffer containing 16 mg/ml of polyethylene glycol (PEG). In contrast, measurements of VTG made by LC/ESI‐MS/MS were unaffected by freezing and, conceptually, are independent from enzymatic degradation. Although the use of aprotinin and PEG effectively reduced the influence of enzymatic and physical degradation caused by freezing and thawing on VTG measurements made by ELISA, it did not improve agreement between the three analytical techniques evaluated. More information is needed regarding the molecular structure and the existence of possible multiple forms of VTG before this protein can be measured adequately in FHM.</description><identifier>ISSN: 0730-7268</identifier><identifier>EISSN: 1552-8618</identifier><identifier>DOI: 10.1897/05-368R.1</identifier><identifier>PMID: 16519293</identifier><identifier>CODEN: ETOCDK</identifier><language>eng</language><publisher>Hoboken: Wiley Periodicals, Inc</publisher><subject>Animal, plant and microbial ecology ; Animals ; Antibodies ; Applied ecology ; Aprotinin ; Biological and medical sciences ; Blood ; Chromatography ; Chromatography, Liquid ; Citrates ; Cryopreservation ; Cyprinidae - physiology ; Degradation ; Ecotoxicology, biological effects of pollution ; ELISA ; Environmental impact ; Environmental science ; Enzyme-linked immunoassay ; Enzyme-Linked Immunosorbent Assay ; Enzymes ; Fathead minnow ; Female ; Fish ; Freeze thaw cycles ; Freezing ; Frozen ; Fundamental and applied biological sciences. Psychology ; General aspects ; Immunoassay ; Ionization ; Liquid chromatography ; Male ; Mass Spectrometry ; Mathematical analysis ; Melting ; Molecular Structure ; Pimephales promelas ; Plasma ; Polyethylene glycol ; Presses ; Reproducibility of Results ; Sampling ; Sensitivity and Specificity ; Thawing ; Toxicology ; Vitellogenin ; Vitellogenins - blood ; Vitellogenins - chemistry</subject><ispartof>Environmental toxicology and chemistry, 2006-02, Vol.25 (2), p.337-348</ispartof><rights>Copyright © 2006 SETAC</rights><rights>2006 INIST-CNRS</rights><rights>Copyright Alliance Communications Group, A Division of Allen Press, Inc. Feb 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5175-219ba94793758ddf2c6949e60f56520f6d6fe3df2d864f1d9f99a1af1342d9913</citedby><cites>FETCH-LOGICAL-c5175-219ba94793758ddf2c6949e60f56520f6d6fe3df2d864f1d9f99a1af1342d9913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17433348$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16519293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brodeur, Julie C.</creatorcontrib><creatorcontrib>Woodburn, Kent B.</creatorcontrib><creatorcontrib>Zhang, Fagen</creatorcontrib><creatorcontrib>Bartels, Michael J.</creatorcontrib><creatorcontrib>Klecka, Gary M.</creatorcontrib><title>Plasma sampling and freezing procedures influence vitellogenin measurements by enzyme-linked immunoassay in the fathead minnow (Pimephales promelas)</title><title>Environmental toxicology and chemistry</title><addtitle>Environmental Toxicology and Chemistry</addtitle><description>The present study compared three different methods for measuring plasma vitellogenin (VTG) in fathead minnow (FHM; Pimephales promelas): A procedure using liquid chromatography with electrospray ionization combined with tandem mass spec‐trometry (LC/ESI‐MS/MS), and two commercial enzyme‐linked immunoassay (ELISA) kits using either anti‐carp or anti‐FHM antibodies. The influence on plasma VTG measurements of using the protease‐inhibitor aprotinin during blood sampling and of submitting the plasma samples to a freeze–thaw cycle before analysis also was evaluated. The addition of aprotinin to the blood during sampling significantly reduced the plasma VTG concentrations measured by ELISA, whereas the VTG values measured after plasma samples were submitted to a freeze–thaw cycle were significantly higher than those measured before freezing. This inflating effect of freezing on VTG measurements made by ELISA could be prevented if plasma samples were frozen diluted in citrate buffer containing 16 mg/ml of polyethylene glycol (PEG). In contrast, measurements of VTG made by LC/ESI‐MS/MS were unaffected by freezing and, conceptually, are independent from enzymatic degradation. Although the use of aprotinin and PEG effectively reduced the influence of enzymatic and physical degradation caused by freezing and thawing on VTG measurements made by ELISA, it did not improve agreement between the three analytical techniques evaluated. More information is needed regarding the molecular structure and the existence of possible multiple forms of VTG before this protein can be measured adequately in FHM.</description><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Applied ecology</subject><subject>Aprotinin</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Chromatography</subject><subject>Chromatography, Liquid</subject><subject>Citrates</subject><subject>Cryopreservation</subject><subject>Cyprinidae - physiology</subject><subject>Degradation</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>ELISA</subject><subject>Environmental impact</subject><subject>Environmental science</subject><subject>Enzyme-linked immunoassay</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Enzymes</subject><subject>Fathead minnow</subject><subject>Female</subject><subject>Fish</subject><subject>Freeze thaw cycles</subject><subject>Freezing</subject><subject>Frozen</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Immunoassay</subject><subject>Ionization</subject><subject>Liquid chromatography</subject><subject>Male</subject><subject>Mass Spectrometry</subject><subject>Mathematical analysis</subject><subject>Melting</subject><subject>Molecular Structure</subject><subject>Pimephales promelas</subject><subject>Plasma</subject><subject>Polyethylene glycol</subject><subject>Presses</subject><subject>Reproducibility of Results</subject><subject>Sampling</subject><subject>Sensitivity and Specificity</subject><subject>Thawing</subject><subject>Toxicology</subject><subject>Vitellogenin</subject><subject>Vitellogenins - blood</subject><subject>Vitellogenins - chemistry</subject><issn>0730-7268</issn><issn>1552-8618</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqNkstu1TAQhiMEoqeFBS-ALCQuXaT4EtvxElVtQZRSEJSl5ROPW7exc4gTSvocPDCOzhGVkLhsbHn8zf-Px1MUjwjeI7WSLzEvmag_7pE7xYJwTstakPpuscCS4VJSUW8V2yldYkyEUup-sUUEJ4oqtih-nLYmBYOSCavWx3NkokWuB7iZD6u-a8COPSTko2tHiA2gb36Atu3OIfqIApiU7wPEIaHlhCDeTAHKLHUFFvkQxtiZlMyUBdBwAciZvBqLgo-xu0YvTn2A1YVps0V2C5DL2X1Q3HOmTfBws-8Unw8PPu2_Lo_fH73Zf3VcNpxIXlKilkZVUjHJa2sdbYSqFAjsuOAUO2GFA5bjthaVI1Y5pQwxjrCKWqUI2ymer3Wz89cR0qCDT01-nInQjUnLijGpuJzJZ38lhZSUYPFvkBKWi6n_B8SVrInI4JPfwMtu7GPuy8wwzjDhGdpdQ03fpdSD06veB9NPmmA9z4jGXM8zomfnxxvBcRnA3pKbocjA0w1gUmNa15vY-HTLzW1hVZ25as1d-xamPzvqDHFBMeWY4rnWcp3m0wDff6WZ_iq3MX-l_nJypA_fneGztycftGQ_Aaib44U</recordid><startdate>200602</startdate><enddate>200602</enddate><creator>Brodeur, Julie C.</creator><creator>Woodburn, Kent B.</creator><creator>Zhang, Fagen</creator><creator>Bartels, Michael J.</creator><creator>Klecka, Gary M.</creator><general>Wiley Periodicals, Inc</general><general>SETAC</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>L6V</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>F1W</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>H97</scope><scope>7X8</scope><scope>7SU</scope><scope>KR7</scope></search><sort><creationdate>200602</creationdate><title>Plasma sampling and freezing procedures influence vitellogenin measurements by enzyme-linked immunoassay in the fathead minnow (Pimephales promelas)</title><author>Brodeur, Julie C. ; Woodburn, Kent B. ; Zhang, Fagen ; Bartels, Michael J. ; Klecka, Gary M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5175-219ba94793758ddf2c6949e60f56520f6d6fe3df2d864f1d9f99a1af1342d9913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Applied ecology</topic><topic>Aprotinin</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Chromatography</topic><topic>Chromatography, Liquid</topic><topic>Citrates</topic><topic>Cryopreservation</topic><topic>Cyprinidae - physiology</topic><topic>Degradation</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>ELISA</topic><topic>Environmental impact</topic><topic>Environmental science</topic><topic>Enzyme-linked immunoassay</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Enzymes</topic><topic>Fathead minnow</topic><topic>Female</topic><topic>Fish</topic><topic>Freeze thaw cycles</topic><topic>Freezing</topic><topic>Frozen</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Immunoassay</topic><topic>Ionization</topic><topic>Liquid chromatography</topic><topic>Male</topic><topic>Mass Spectrometry</topic><topic>Mathematical analysis</topic><topic>Melting</topic><topic>Molecular Structure</topic><topic>Pimephales promelas</topic><topic>Plasma</topic><topic>Polyethylene glycol</topic><topic>Presses</topic><topic>Reproducibility of Results</topic><topic>Sampling</topic><topic>Sensitivity and Specificity</topic><topic>Thawing</topic><topic>Toxicology</topic><topic>Vitellogenin</topic><topic>Vitellogenins - blood</topic><topic>Vitellogenins - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brodeur, Julie C.</creatorcontrib><creatorcontrib>Woodburn, Kent B.</creatorcontrib><creatorcontrib>Zhang, Fagen</creatorcontrib><creatorcontrib>Bartels, Michael J.</creatorcontrib><creatorcontrib>Klecka, Gary M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest research library</collection><collection>ProQuest Science Journals</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>MEDLINE - Academic</collection><collection>Environmental Engineering Abstracts</collection><collection>Civil Engineering Abstracts</collection><jtitle>Environmental toxicology and chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brodeur, Julie C.</au><au>Woodburn, Kent B.</au><au>Zhang, Fagen</au><au>Bartels, Michael J.</au><au>Klecka, Gary M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasma sampling and freezing procedures influence vitellogenin measurements by enzyme-linked immunoassay in the fathead minnow (Pimephales promelas)</atitle><jtitle>Environmental toxicology and chemistry</jtitle><addtitle>Environmental Toxicology and Chemistry</addtitle><date>2006-02</date><risdate>2006</risdate><volume>25</volume><issue>2</issue><spage>337</spage><epage>348</epage><pages>337-348</pages><issn>0730-7268</issn><eissn>1552-8618</eissn><coden>ETOCDK</coden><abstract>The present study compared three different methods for measuring plasma vitellogenin (VTG) in fathead minnow (FHM; Pimephales promelas): A procedure using liquid chromatography with electrospray ionization combined with tandem mass spec‐trometry (LC/ESI‐MS/MS), and two commercial enzyme‐linked immunoassay (ELISA) kits using either anti‐carp or anti‐FHM antibodies. The influence on plasma VTG measurements of using the protease‐inhibitor aprotinin during blood sampling and of submitting the plasma samples to a freeze–thaw cycle before analysis also was evaluated. The addition of aprotinin to the blood during sampling significantly reduced the plasma VTG concentrations measured by ELISA, whereas the VTG values measured after plasma samples were submitted to a freeze–thaw cycle were significantly higher than those measured before freezing. This inflating effect of freezing on VTG measurements made by ELISA could be prevented if plasma samples were frozen diluted in citrate buffer containing 16 mg/ml of polyethylene glycol (PEG). In contrast, measurements of VTG made by LC/ESI‐MS/MS were unaffected by freezing and, conceptually, are independent from enzymatic degradation. Although the use of aprotinin and PEG effectively reduced the influence of enzymatic and physical degradation caused by freezing and thawing on VTG measurements made by ELISA, it did not improve agreement between the three analytical techniques evaluated. More information is needed regarding the molecular structure and the existence of possible multiple forms of VTG before this protein can be measured adequately in FHM.</abstract><cop>Hoboken</cop><pub>Wiley Periodicals, Inc</pub><pmid>16519293</pmid><doi>10.1897/05-368R.1</doi><tpages>12</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0730-7268 |
ispartof | Environmental toxicology and chemistry, 2006-02, Vol.25 (2), p.337-348 |
issn | 0730-7268 1552-8618 |
language | eng |
recordid | cdi_proquest_miscellaneous_743379571 |
source | Wiley-Blackwell Read & Publish Collection |
subjects | Animal, plant and microbial ecology Animals Antibodies Applied ecology Aprotinin Biological and medical sciences Blood Chromatography Chromatography, Liquid Citrates Cryopreservation Cyprinidae - physiology Degradation Ecotoxicology, biological effects of pollution ELISA Environmental impact Environmental science Enzyme-linked immunoassay Enzyme-Linked Immunosorbent Assay Enzymes Fathead minnow Female Fish Freeze thaw cycles Freezing Frozen Fundamental and applied biological sciences. Psychology General aspects Immunoassay Ionization Liquid chromatography Male Mass Spectrometry Mathematical analysis Melting Molecular Structure Pimephales promelas Plasma Polyethylene glycol Presses Reproducibility of Results Sampling Sensitivity and Specificity Thawing Toxicology Vitellogenin Vitellogenins - blood Vitellogenins - chemistry |
title | Plasma sampling and freezing procedures influence vitellogenin measurements by enzyme-linked immunoassay in the fathead minnow (Pimephales promelas) |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T12%3A56%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plasma%20sampling%20and%20freezing%20procedures%20influence%20vitellogenin%20measurements%20by%20enzyme-linked%20immunoassay%20in%20the%20fathead%20minnow%20(Pimephales%20promelas)&rft.jtitle=Environmental%20toxicology%20and%20chemistry&rft.au=Brodeur,%20Julie%20C.&rft.date=2006-02&rft.volume=25&rft.issue=2&rft.spage=337&rft.epage=348&rft.pages=337-348&rft.issn=0730-7268&rft.eissn=1552-8618&rft.coden=ETOCDK&rft_id=info:doi/10.1897/05-368R.1&rft_dat=%3Cproquest_cross%3E67721061%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c5175-219ba94793758ddf2c6949e60f56520f6d6fe3df2d864f1d9f99a1af1342d9913%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=210353015&rft_id=info:pmid/16519293&rfr_iscdi=true |