Purification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye

• Published in: Biotechnology letters 2008-12, Vol.30 (12), p.2091-2096
• Main Authors: Guo, Mei, Lu, Fuping, Liu, Minyao, Li, Tuoping, Pu, Jun, Wang, Na, Liang, Peng, Zhang, Chenyun
• Format: Article
• Language: English
• Subjects: Anthraquinones - chemistry; Applied Microbiology; Basidiomycota - enzymology; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biophysics; Biotechnology; Chromatography, Gel; Chromatography, Ion Exchange; Coloring Agents - chemistry; Dyes; Enzymes; Fundamental and applied biological sciences. Psychology; Fungi; Laccase - isolation & purification; Laccase - metabolism; Life Sciences; Microbiology; Original Research Paper; Pichia - genetics; Pichia methanolica; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Trametes versicolor; Ultrafiltration
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-008-9817-z

A recombinant laccase from Trametes versicolor in Pichia methanolica was produced constitutively in a defined medium. The recombinant laccase was purified using ultrafiltration, anion-exchange chromatography, and gel filtration. The molecular weight of the purified laccase was estimated as 64 kDa by SDS-PAGE. The purified recombinant laccase decolorized more than 90% of Remazol Brilliant Blue R (RBBR) initially at 80 mg l⁻¹ after 16 h at 45°C and pH 5 when 25 U laccase ml⁻¹ was used. The purified recombinant laccase could efficiently decolorize RBBR without additional redox mediators.