Production of deuterated zeaxanthin by Flavobacterium multivorum and its detection by resonance raman and mass spectrometric methods

Flavobacterium multivorum, a zeaxanthin-producing organism, was grown aerobically in a medium prepared with deuterated water. Atmospheric pressure chemical ionization mass spectrometry (APCI-MS) and resonance Raman spectroscopy (RRS) analysis revealed approximately 75% replacement of hydrogen by deu...

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Bibliographic Details
Published in:Biotechnology letters 2005-11, Vol.27 (21), p.1719-1723
Main Authors: BHOSALE, Prakash, TEREDESAI, Pallavi V, JIN LIHONG, ERMAKOV, Igor V, GELLERMANN, Werner, BERNSTEIN, Paul S
Format: Article
Language:English
Subjects:
Atmospheric pressure
beta Carotene - analogs & derivatives
beta Carotene - biosynthesis
beta Carotene - chemistry
Biological and medical sciences
Biotechnology
Deuterium
Deuterium Oxide - metabolism
Flavobacterium
Flavobacterium - chemistry
Flavobacterium - growth & development
Flavobacterium multivorum
Fundamental and applied biological sciences. Psychology
Ionization
Liquid chromatography
Mass spectrometry
Resonance
Spectrometry, Mass, Electrospray Ionization
Spectrum Analysis, Raman
Xanthophylls
Zeaxanthins
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Summary:Flavobacterium multivorum, a zeaxanthin-producing organism, was grown aerobically in a medium prepared with deuterated water. Atmospheric pressure chemical ionization mass spectrometry (APCI-MS) and resonance Raman spectroscopy (RRS) analysis revealed approximately 75% replacement of hydrogen by deuterium atoms as indicated by the molecular mass cluster at around m/z 600. Deuterated zeaxanthin upon excitation with a 488 nm laser exhibited characteristic resonance Raman vibrational modes at 1,161 and 1,504 cm(-1) as compared to 1,007, 1,159 and 1,525 cm(-1 )for undeuterated zeaxanthin. HPLC/APCI-MS and HPLC/RRS were specific and sensitive with limits of detection of 2.5 pg and 50 ng, respectively.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-005-2737-2