Preparation of corn (Zea mays) peptides and their protective effect against alcohol-induced acute hepatic injury in NH mice

CPS [corn (Zea mays) peptides] were prepared from corn gluten meal by proteolysis with alcalase, an alkaline protease. The molecular‐mass distribution of CPS is from 200 to 1000 Da as determined by MS. The amino acid composition of CPS was also analysed by HPLC. CPS contains almost no free amino aci...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and applied biochemistry 2007-07, Vol.47 (3), p.169-174
Main Authors: Li, Hong-Mei, Guo, Ping, Hu, Xin, Xu, Li, Zhang, Xue-Zhong
Format: Article
Language:English
Subjects:
Acute Disease
acute hepatic injury
alcalase
alcohol
Animals
autoxidation
Biological and medical sciences
Biotechnology
corn (Zea mays) peptide
Ethanol - poisoning
Fundamental and applied biological sciences. Psychology
lipid peroxidation
Liver Diseases, Alcoholic - etiology
Liver Diseases, Alcoholic - pathology
Liver Diseases, Alcoholic - prevention & control
Mice
Peptides - therapeutic use
Plant Extracts - therapeutic use
Plant Proteins - therapeutic use
Treatment Outcome
Zea mays
Zea mays - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:CPS [corn (Zea mays) peptides] were prepared from corn gluten meal by proteolysis with alcalase, an alkaline protease. The molecular‐mass distribution of CPS is from 200 to 1000 Da as determined by MS. The amino acid composition of CPS was also analysed by HPLC. CPS contains almost no free amino acids. The protective effect of CPS against acute hepatic injuries induced by alcohol was verified in NH mice that were fed with different dosages of CPS for 30 days and subsequently given an acute dose of alcohol orally. As a result, CPS reduced both hepatic malondialdehyde and triacylglycerol levels, along with enhanced hepatic GSH (glutathione) levels, relative to the control. Hepatic histological changes were also observed. The result indicates that CPS is capable of attenuating ethanol‐induced hepatic injury. The effect of CPS on removing superoxide anion in vitro was also studied as an additional proof that CPS is capable of abating hepatic superoxidant stress.
ISSN:0885-4513
1470-8744
DOI:10.1042/BA20060183