RopGAP4-Dependent Rop GTPase Rheostat Control of Arabidopsis Oxygen Deprivation Tolerance

• Published in: Science (American Association for the Advancement of Science) 2002-06, Vol.296 (5575), p.2026-2028
• Main Authors: Baxter-Burrell, Airica, Yang, Zhenbiao, Springer, Patricia S., Bailey-Serres, Julia
• Format: Article
• Language: English
• Subjects: ADH gene; Alcohol Dehydrogenase - genetics; Alcohol Dehydrogenase - metabolism; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Biological and medical sciences; Caffeine - pharmacology; Calcium - metabolism; Cytosol - metabolism; DNA Transposable Elements; Feedback, Physiological; Fermentation; Flowers & plants; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; Genes; Guanosine Triphosphate - metabolism; Hydrogen Peroxide - metabolism; Logical Thinking; Messenger RNA; Metabolism; Molecular biology; NADPH oxidase; NADPH Oxidases - metabolism; Negative feedback; Onium Compounds - pharmacology; Oxidases; Oxidative Stress; Oxygen; Oxygen - physiology; Photosynthesis, respiration. Anabolism, catabolism; Physiological aspects; Plant cells; Plant physiology; Plant physiology and development; Plants; rho GTP-Binding Proteins - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Plant - genetics; RNA, Plant - metabolism; Rop protein; ROPGAP4 protein; Second Messenger Systems; Seedlings; Signal Transduction; Transcription, Genetic
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1071505

Transient soil flooding limits cellular oxygen to roots and reduces crop yield. Plant response to oxygen deprivation involves increased expression of the alcohol dehydrogenase gene (ADH) and ethanolic fermentation. Disruption of the Arabidopsis gene that encodes Rop (RHO-like small G protein of plants) guanosine triphosphatase (GTPase) activating protein 4 (ROPGAP4), a Rop deactivator, elevates ADH expression in response to oxygen deprivation but decreases tolerance to stress. Rop-dependent production of hydrogen peroxide via a diphenylene iodonium chloride-sensitive calcium-dependent reduced nicotinamide adenine dinucleotide phosphatase (NADPH) oxidase is necessary for induction of both ADH and RopGAP4 expression. Tolerance to oxygen deprivation requires Rop activation and RopGAP4-dependent negative feedback regulation. This Rop signal transduction rheostat balances the ability to increase ethanolic fermentation with survival.