Rational strategy for the production of new crude lipases from Candida rugosa

• Published in: Biotechnology letters 2005-04, Vol.27 (7), p.499-503
• Main Authors: DOMINGUEZ DE MARIA, Pablo, SANCHEZ-MONTERO, José M, ALCANTARA, Andrés R, VALERO, Francisco, SINISTERRA, José V
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; Candida - enzymology; Candida rugosa; Carbon sources; Dodecanol - metabolism; Enzyme Induction; Fermentation; Fundamental and applied biological sciences. Psychology; Glycerol - metabolism; Helianthus; Isoenzymes - chemistry; Isoenzymes - isolation & purification; Isoenzymes - metabolism; Lipase - chemistry; Lipase - isolation & purification; Lipase - metabolism; Microbiology; Olea; Oleic Acid - metabolism; Oleic Acids - metabolism; Olive Oil; Plant Oils - metabolism; Sunflower Oil
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-005-2540-0

Candida rugosa was cultured using different inducers (oleic acid, olive oil, sunflower oil, n-dodecanol and glycerol) as the only carbon source in batch conditions, as well as in several fed-batch fermentations (oleic acid as inducer) at variable feed rate conditions. The N-terminal analysis of each crude lipase revealed that, while the isoenzymes Lip2 and Lip3 are always secreted (at different proportions depending on the inducer), Lip1 was produced only using n-dodecanol (batch conditions) or oleic acid (fed-batch at high feed rate). The nature of the inducer controls the isoenzyme percentage; when this is fixed, as well as the feed rate in fed-batch fermentation, the isoenzymatic profile remained unaltered and the samples differed only in the activity of the lipases, as determined by heptyl oleate synthesis.