Scale-up micropropagation of sweet basil (Ocimum basilicum L.) in an airlift bioreactor and accumulation of rosmarinic acid

Nodal explants with lateral buds and leaf-derived suspension cultures of sweet basil, Ocimum basilicum L., were cultured in 5 l airlift bioreactors for three weeks, thereby increasing the fresh wt of suspension cultures 2.5-fold. Rosmarinic acid accumulated at 29 micromicrog g(-1) dry wt in the susp...

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Bibliographic Details
Published in:Biotechnology letters 2004-03, Vol.26 (6), p.521-523
Main Authors: KINTZIOS, Spiridon, KOLLIAS, Haralambos, STRAITOURIS, Evangelos, MAKRI, Olga
Format: Article
Language:English
Subjects:
Antioxidants - metabolism
Biological and medical sciences
Bioreactors
Biotechnology
Cinnamates - metabolism
Depsides
Fundamental and applied biological sciences. Psychology
Herbs
Ocimum basilicum
Ocimum basilicum - metabolism
Plant Leaves - growth & development
Plant Leaves - metabolism
Rosmarinic Acid
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Summary:Nodal explants with lateral buds and leaf-derived suspension cultures of sweet basil, Ocimum basilicum L., were cultured in 5 l airlift bioreactors for three weeks, thereby increasing the fresh wt of suspension cultures 2.5-fold. Rosmarinic acid accumulated at 29 micromicrog g(-1) dry wt in the suspension culture but, for micropropagated plants, it reached 178 microg g(-1) dry wt.
ISSN:0141-5492
1573-6776
DOI:10.1023/B:BILE.0000019561.89044.30