Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles

• Published in: Science (American Association for the Advancement of Science) 2000-02, Vol.287 (5454), p.873-880
• Main Authors: Roberts, Christopher J., Nelson, Bryce, Marton, Matthew J., Stoughton, Roland, Meyer, Michael R., Bennett, Holly A., He, Yudong D., Dai, Hongyue, Walker, Wynn L., Hughes, Timothy R., Tyers, Mike, Boone, Charles, Friend, Stephen H.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Cell cycle; Cell Cycle Proteins; Cell growth; Cell physiology; Cells; Chromosome mapping; Cyclin-Dependent Kinase Inhibitor Proteins; Diploidy; Feedback (Response); Fundamental and applied biological sciences. Psychology; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungal Proteins - physiology; Fus3 protein; G1 Phase; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Fungal; Genes; Genetic regulation; Genetic transcription; Genetics; Genome, Fungal; Genomics; Haploidy; Kss1 protein; Lipoproteins - pharmacology; Lipoproteins - physiology; MAP Kinase Signaling System; Mating Factor; Mitogen-Activated Protein Kinases - metabolism; Molecular and cellular biology; Multigene Family; Oligonucleotide Array Sequence Analysis; Peptides - pharmacology; Peptides - physiology; Pheromones; Profiles; Protein Kinase C - metabolism; Protein kinases; Proteins; Repressor Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - physiology; Saccharomyces cerevisiae Proteins; Signal transduction; T lymphocytes; Transcription (Genetics); Transcription Factors - metabolism; Transcriptional Activation; Transcripts (Written Records); Yeast; Yeasts
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.287.5454.873

Genome-wide transcript profiling was used to monitor signal transduction during yeast pheromone response. Genetic manipulations allowed analysis of changes in gene expression underlying pheromone signaling, cell cycle control, and polarized morphogenesis. A two-dimensional hierarchical clustered matrix, covering 383 of the most highly regulated genes, was constructed from 46 diverse experimental conditions. Diagnostic subsets of coexpressed genes reflected signaling activity, cross talk, and overlap of multiple mitogen-activated protein kinase (MAPK) pathways. Analysis of the profiles specified by two different MAPKs-Fus3p and Kss1p-revealed functional overlap of the filamentous growth and mating responses. Global transcript analysis reflects biological responses associated with the activation and perturbation of signal transduction pathways.