Transport of dsRNA into Cells by the Transmembrane Protein SID-1

RNA interference (RNAi) spreads systemically in plants and nematodes to silence gene expression distant from the site of initiation. We previously identified a gene, sid-1, essential for systemic but not cell-autonomous RNAi in Caenorhabditis elegans. Here, we demonstrate that SID-1 is a multispan t...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2003-09, Vol.301 (5639), p.1545-1547
Main Authors: Feinberg, Evan H., Hunter, Craig P.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Amino acids
Animals
Biological and medical sciences
Biological Transport
Caenorhabditis elegans
Caenorhabditis elegans - genetics
Caenorhabditis elegans Proteins - chemistry
Caenorhabditis elegans Proteins - metabolism
Cell Line
Cell lines
Cell membranes
Cell physiology
Daughter cells
Diffusion
Double stranded RNA
Drosophila
Flowers & plants
Fundamental and applied biological sciences. Psychology
Genetic aspects
Genetics
Germ cells
Membrane and intracellular transports
Membrane proteins
Membrane Proteins - chemistry
Membrane Proteins - metabolism
Molecular and cellular biology
P branes
Physiological aspects
Proteins
Recombinant Proteins - metabolism
Research Design
Ribonucleic acid
RNA
RNA Interference
RNA, Double-Stranded - chemistry
RNA, Double-Stranded - genetics
RNA, Double-Stranded - metabolism
Small interfering RNA
Stem cells
Topology
Transfection
Transmembrane proteins
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Summary:RNA interference (RNAi) spreads systemically in plants and nematodes to silence gene expression distant from the site of initiation. We previously identified a gene, sid-1, essential for systemic but not cell-autonomous RNAi in Caenorhabditis elegans. Here, we demonstrate that SID-1 is a multispan transmembrane protein that sensitizes Drosophila cells to soaking RNAi with a potency that is dependent on double-stranded RNA (dsRNA) length. Further analyses revealed that SID-1 enables passive cellular uptake of dsRNA. These data indicate that systemic RNAi in C. elegans involves SID-1-mediated intercellular transport of dsRNA.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1087117