Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation

Recently, we showed that antibodies catalyze the generation of hydrogen peroxide (H2O2) from singlet molecular oxygen ($^{1}O_{2}^*$) and water. Here, we show that this process can lead to efficient killing of bacteria, regardless of the antigen specificity of the antibody. H2O2production by antibod...

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Published in:Science (American Association for the Advancement of Science) 2002-12, Vol.298 (5601), p.2195-2199
Main Authors: Wentworth, Paul, McDunn, Jonathan E., Wentworth, Anita D., Takeuchi, Cindy, Nieva, Jorge, Jones, Teresa, Bautista, Cristina, Ruedi, Julie M., Gutierrez, Abel, Janda, Kim D., Babior, Bernard M., Eschenmoser, Albert, Lerner, Richard A.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Catalytic - immunology
Antibodies, Catalytic - metabolism
Antigens
Arthus Reaction - immunology
Arthus Reaction - metabolism
Bacteria
Biological and medical sciences
Blood Bactericidal Activity
Catalase - metabolism
Catalysis
Escherichia coli - immunology
Fundamental and applied biological sciences. Psychology
Hematoporphyrins - metabolism
Humans
Hydrogen Peroxide - metabolism
Immunology
Indigo Carmine - metabolism
Inflammation - immunology
Inflammation - metabolism
Irradiation
Light
Mechanisms. Catalysis. Electron transfer. Models
Mice
Molecular biophysics
Neutrophil Activation
Neutrophils
Neutrophils - immunology
Neutrophils - metabolism
Oxidation
Oxidation-Reduction
Ozone
Ozone - metabolism
Physical chemistry in biology
Rabbits
Rats
Rats, Sprague-Dawley
Respiratory Burst
Signatures
Singlet Oxygen - metabolism
Sulfonic acids
Ultraviolet Rays
Viral antibodies
Water
Water - metabolism
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container_issue 5601
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container_title Science (American Association for the Advancement of Science)
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creator Wentworth, Paul
McDunn, Jonathan E.
Wentworth, Anita D.
Takeuchi, Cindy
Nieva, Jorge
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Bautista, Cristina
Ruedi, Julie M.
Gutierrez, Abel
Janda, Kim D.
Babior, Bernard M.
Eschenmoser, Albert
Lerner, Richard A.
description Recently, we showed that antibodies catalyze the generation of hydrogen peroxide (H2O2) from singlet molecular oxygen ($^{1}O_{2}^*$) and water. Here, we show that this process can lead to efficient killing of bacteria, regardless of the antigen specificity of the antibody. H2O2production by antibodies alone was found to be not sufficient for bacterial killing. Our studies suggested that the antibody-catalyzed water-oxidation pathway produced an additional molecular species with a chemical signature similar to that of ozone. This species is also generated during the oxidative burst of activated human neutrophils and during inflammation. These observations suggest that alternative pathways may exist for biological killing of bacteria that are mediated by potent oxidants previously unknown to biology.
doi_str_mv 10.1126/science.1077642
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Geoastrophysical Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wentworth, Paul</au><au>McDunn, Jonathan E.</au><au>Wentworth, Anita D.</au><au>Takeuchi, Cindy</au><au>Nieva, Jorge</au><au>Jones, Teresa</au><au>Bautista, Cristina</au><au>Ruedi, Julie M.</au><au>Gutierrez, Abel</au><au>Janda, Kim D.</au><au>Babior, Bernard M.</au><au>Eschenmoser, Albert</au><au>Lerner, Richard A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2002-12-13</date><risdate>2002</risdate><volume>298</volume><issue>5601</issue><spage>2195</spage><epage>2199</epage><pages>2195-2199</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Recently, we showed that antibodies catalyze the generation of hydrogen peroxide (H2O2) from singlet molecular oxygen ($^{1}O_{2}^*$) and water. Here, we show that this process can lead to efficient killing of bacteria, regardless of the antigen specificity of the antibody. H2O2production by antibodies alone was found to be not sufficient for bacterial killing. Our studies suggested that the antibody-catalyzed water-oxidation pathway produced an additional molecular species with a chemical signature similar to that of ozone. This species is also generated during the oxidative burst of activated human neutrophils and during inflammation. These observations suggest that alternative pathways may exist for biological killing of bacteria that are mediated by potent oxidants previously unknown to biology.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>12434011</pmid><doi>10.1126/science.1077642</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 0036-8075
ispartof Science (American Association for the Advancement of Science), 2002-12, Vol.298 (5601), p.2195-2199
issn 0036-8075
1095-9203
language eng
recordid cdi_proquest_miscellaneous_743546538
source American Association for the Advancement of Science; JSTOR Archival Journals and Primary Sources Collection; Social Science Premium Collection; Alma/SFX Local Collection; Education Collection
subjects Animals
Antibodies
Antibodies, Catalytic - immunology
Antibodies, Catalytic - metabolism
Antigens
Arthus Reaction - immunology
Arthus Reaction - metabolism
Bacteria
Biological and medical sciences
Blood Bactericidal Activity
Catalase - metabolism
Catalysis
Escherichia coli - immunology
Fundamental and applied biological sciences. Psychology
Hematoporphyrins - metabolism
Humans
Hydrogen Peroxide - metabolism
Immunology
Indigo Carmine - metabolism
Inflammation - immunology
Inflammation - metabolism
Irradiation
Light
Mechanisms. Catalysis. Electron transfer. Models
Mice
Molecular biophysics
Neutrophil Activation
Neutrophils
Neutrophils - immunology
Neutrophils - metabolism
Oxidation
Oxidation-Reduction
Ozone
Ozone - metabolism
Physical chemistry in biology
Rabbits
Rats
Rats, Sprague-Dawley
Respiratory Burst
Signatures
Singlet Oxygen - metabolism
Sulfonic acids
Ultraviolet Rays
Viral antibodies
Water
Water - metabolism
title Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation
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