UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography

Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different m...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2010, Vol.21 (1), p.178-190
Main Authors: Bultel, Laurent, Landoni, Malena, Grand, Eric, Couto, Alicia S., Kovensky, José
Format: Article
Language:English
Subjects:
Acids
Analytical Chemistry
Anion exchanging
Anticoagulants
Bioinformatics
Biotechnology
Carbohydrate Sequence
Chemistry
Chemistry and Materials Science
Chromatography
Chromatography, High Pressure Liquid - economics
Chromatography, High Pressure Liquid - methods
Chromatography, Ion Exchange - economics
Chromatography, Ion Exchange - methods
Degradation
Desorption
Dihydroxybenzoic acid
Heparin - chemistry
Hydroxycinnamic acid
Ionization
Ions
Mass spectrometry
Molecular Sequence Data
Molecular Structure
Nitrous acid
Nitrous Acid - chemistry
Oligosaccharides
Oligosaccharides - analysis
Organic Chemistry
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - economics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Spectrophotometry, Ultraviolet
Structural analysis
Time Factors
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Summary:Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis. Sulfated oligosaccharides obtained by nitrous acid degradation of heparin and HPAEC separation were analyzed by UV-MALDI-TOF using CHCA, nor-harmane and DHB matrices.
ISSN:1044-0305
1879-1123
DOI:10.1016/j.jasms.2009.09.025