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UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different m...
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| Published in: | Journal of the American Society for Mass Spectrometry 2010, Vol.21 (1), p.178-190 |
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| cites | cdi_FETCH-LOGICAL-c508t-8c03329940aa010f143ddac7436253c2bb3b271c2026ff71ebe9c1572919e2a13 |
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| container_start_page | 178 |
| container_title | Journal of the American Society for Mass Spectrometry |
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| creator | Bultel, Laurent Landoni, Malena Grand, Eric Couto, Alicia S. Kovensky, José |
| description | Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis.
Sulfated oligosaccharides obtained by nitrous acid degradation of heparin and HPAEC separation were analyzed by UV-MALDI-TOF using CHCA, nor-harmane and DHB matrices. |
| doi_str_mv | 10.1016/j.jasms.2009.09.025 |
| format | article |
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Sulfated oligosaccharides obtained by nitrous acid degradation of heparin and HPAEC separation were analyzed by UV-MALDI-TOF using CHCA, nor-harmane and DHB matrices.</description><identifier>ISSN: 1044-0305</identifier><identifier>EISSN: 1879-1123</identifier><identifier>DOI: 10.1016/j.jasms.2009.09.025</identifier><identifier>PMID: 19892568</identifier><language>eng</language><publisher>New York: Elsevier Inc</publisher><subject>Acids ; Analytical Chemistry ; Anion exchanging ; Anticoagulants ; Bioinformatics ; Biotechnology ; Carbohydrate Sequence ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Chromatography, High Pressure Liquid - economics ; Chromatography, High Pressure Liquid - methods ; Chromatography, Ion Exchange - economics ; Chromatography, Ion Exchange - methods ; Degradation ; Desorption ; Dihydroxybenzoic acid ; Heparin - chemistry ; Hydroxycinnamic acid ; Ionization ; Ions ; Mass spectrometry ; Molecular Sequence Data ; Molecular Structure ; Nitrous acid ; Nitrous Acid - chemistry ; Oligosaccharides ; Oligosaccharides - analysis ; Organic Chemistry ; Proteomics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - economics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Spectrophotometry, Ultraviolet ; Structural analysis ; Time Factors</subject><ispartof>Journal of the American Society for Mass Spectrometry, 2010, Vol.21 (1), p.178-190</ispartof><rights>2010 American Society for Mass Spectrometry</rights><rights>American Society for Mass Spectrometry 2010</rights><rights>2010 American Society for Mass Spectrometry. Published by Elsevier Inc. All rights reserved.</rights><rights>Journal of The American Society for Mass Spectrometry is a copyright of Springer, 2010.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-8c03329940aa010f143ddac7436253c2bb3b271c2026ff71ebe9c1572919e2a13</citedby><cites>FETCH-LOGICAL-c508t-8c03329940aa010f143ddac7436253c2bb3b271c2026ff71ebe9c1572919e2a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022,27922,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19892568$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bultel, Laurent</creatorcontrib><creatorcontrib>Landoni, Malena</creatorcontrib><creatorcontrib>Grand, Eric</creatorcontrib><creatorcontrib>Couto, Alicia S.</creatorcontrib><creatorcontrib>Kovensky, José</creatorcontrib><title>UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography</title><title>Journal of the American Society for Mass Spectrometry</title><addtitle>J Am Soc Mass Spectrom</addtitle><addtitle>J Am Soc Mass Spectrom</addtitle><description>Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis.
Sulfated oligosaccharides obtained by nitrous acid degradation of heparin and HPAEC separation were analyzed by UV-MALDI-TOF using CHCA, nor-harmane and DHB matrices.</description><subject>Acids</subject><subject>Analytical Chemistry</subject><subject>Anion exchanging</subject><subject>Anticoagulants</subject><subject>Bioinformatics</subject><subject>Biotechnology</subject><subject>Carbohydrate Sequence</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - economics</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, Ion Exchange - economics</subject><subject>Chromatography, Ion Exchange - methods</subject><subject>Degradation</subject><subject>Desorption</subject><subject>Dihydroxybenzoic acid</subject><subject>Heparin - chemistry</subject><subject>Hydroxycinnamic acid</subject><subject>Ionization</subject><subject>Ions</subject><subject>Mass spectrometry</subject><subject>Molecular Sequence Data</subject><subject>Molecular Structure</subject><subject>Nitrous acid</subject><subject>Nitrous Acid - chemistry</subject><subject>Oligosaccharides</subject><subject>Oligosaccharides - analysis</subject><subject>Organic Chemistry</subject><subject>Proteomics</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - economics</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Structural analysis</subject><subject>Time Factors</subject><issn>1044-0305</issn><issn>1879-1123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFUk2P0zAQtRCIXQq_AAlZ4sApxR9xEh84VN1dulKXIrHL1XIcp3WUxMFOEflD_E4mtBKIwyLZskfz3hvPPCP0mpIlJTR73ywbHbu4ZITI5byYeIIuaZHLhFLGn8KdpGlCOBEX6EWMDSE0JzJ_ji6oLCQTWXGJfj58Te5W26vb5H53g-90jPjLYM0YfGfHMOFVr9spuoh9jTd20MH1eNe6vY_amAOElY14V47a9bbC5YQ_OeAeI14ZV-G17yFqW0hd2X3QlR6d77HuK7xx-wP-bEPtQ6d7Y6HSnLr-Aar93uL1AZ6gRw-s4TC9RM9q3Ub76nwu0MPN9f16k2x3H2_Xq21iBCnGpDCEcyZlSrQmlNQ05VWlTZ7yjAluWFnykuXUMMKyus6pLa00VORMUmmZpnyB3p10h-C_HW0cVeeisW2rewtdKVASglCZ_h_JuSQ8h71Ab_9BNv4YYK5RUSmo4FRkM4qfUCb4GIOt1RBcp8OkKFGz36pRv_1Ws99qXtDSAr05ax_LzlZ_OGeDAZCeABFSMNfwV_FHdT-caBaG_d0BLRpnwabKBfgdqvLuUf4v3jPOpA</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Bultel, Laurent</creator><creator>Landoni, Malena</creator><creator>Grand, Eric</creator><creator>Couto, Alicia S.</creator><creator>Kovensky, José</creator><general>Elsevier Inc</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>2010</creationdate><title>UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography</title><author>Bultel, Laurent ; Landoni, Malena ; Grand, Eric ; Couto, Alicia S. ; Kovensky, José</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-8c03329940aa010f143ddac7436253c2bb3b271c2026ff71ebe9c1572919e2a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acids</topic><topic>Analytical Chemistry</topic><topic>Anion exchanging</topic><topic>Anticoagulants</topic><topic>Bioinformatics</topic><topic>Biotechnology</topic><topic>Carbohydrate Sequence</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - 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Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis.
Sulfated oligosaccharides obtained by nitrous acid degradation of heparin and HPAEC separation were analyzed by UV-MALDI-TOF using CHCA, nor-harmane and DHB matrices.</abstract><cop>New York</cop><pub>Elsevier Inc</pub><pmid>19892568</pmid><doi>10.1016/j.jasms.2009.09.025</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Acids Analytical Chemistry Anion exchanging Anticoagulants Bioinformatics Biotechnology Carbohydrate Sequence Chemistry Chemistry and Materials Science Chromatography Chromatography, High Pressure Liquid - economics Chromatography, High Pressure Liquid - methods Chromatography, Ion Exchange - economics Chromatography, Ion Exchange - methods Degradation Desorption Dihydroxybenzoic acid Heparin - chemistry Hydroxycinnamic acid Ionization Ions Mass spectrometry Molecular Sequence Data Molecular Structure Nitrous acid Nitrous Acid - chemistry Oligosaccharides Oligosaccharides - analysis Organic Chemistry Proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - economics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Spectrophotometry, Ultraviolet Structural analysis Time Factors |
| title | UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography |
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