White Collar-1, a Circadian Blue Light Photoreceptor, Binding to the Frequency Promoter

In the fungus Neurospora crassa, the blue light photoreceptor(s) and signaling pathway(s) have not been identified. We examined light signaling by exploiting the light sensitivity of the Neurospora biological clock, specifically the rapid induction by light of the clock component frequency (frq). Li...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2002-08, Vol.297 (5582), p.815-819
Main Authors: Froehlich, Allan C., Liu, Yi, Loros, Jennifer J., Dunlap, Jay C.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Circadian rhythm
Circadian Rhythm - radiation effects
Circadian rhythms
Color
DNA
DNA probes
DNA, Fungal - genetics
DNA, Fungal - metabolism
DNA-Binding Proteins - metabolism
Dose response relationship
Flavin Mononucleotide - metabolism
Flavin-Adenine Dinucleotide - metabolism
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
Fungal Proteins - metabolism
Fungi
Gels
Gene Deletion
Gene Expression Regulation, Fungal - radiation effects
Light
Logical Thinking
Microbiology
Miscellaneous
Mycology
Neurospora
Neurospora crassa - genetics
Neurospora crassa - metabolism
Neurospora crassa - radiation effects
Photophobia
Photoreceptors
Photoreceptors, Microbial - metabolism
Promoter regions
Promoter Regions, Genetic - genetics
Protein Binding
Response Elements - genetics
RNA, Fungal - genetics
RNA, Fungal - metabolism
Signal Transduction
Transcription Factors - metabolism
Transcriptional Activation - radiation effects
Transcripts (Written Records)
Visible spectrum
Wavelengths
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the fungus Neurospora crassa, the blue light photoreceptor(s) and signaling pathway(s) have not been identified. We examined light signaling by exploiting the light sensitivity of the Neurospora biological clock, specifically the rapid induction by light of the clock component frequency (frq). Light induction of frq is transcriptionally controlled and requires two cis-acting elements (LREs) in the frq promoter. Both LREs are bound by a White Collar-1 (WC-1)/White Collar-2 (WC-2)-containing complex (WCC), and light causes decreased mobility of the WCC bound to the LREs. The use of in vitro-translated WC-1 and WC-2 confirmed that WC-1, with flavin adenine dinucleotide as a cofactor, is the blue light photoreceptor that mediates light input to the circadian system through direct binding (with WC-2) to the frq promoter.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1073681