Fluorescence anisotropy: from single molecules to live cells

The polarization of light emitted by fluorescent probes is an easily accessible physical quantity that is related to a multitude of molecular parameters including conformation, orientation, size and the nanoscale environment conditions, such as dynamic viscosity and temperature. In analytical bioche...

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Bibliographic Details
Published in:Analyst (London) 2010-03, Vol.135 (3), p.452-459
Main Authors: GRADINARU, Claudiu C, MARUSHCHAK, Denys O, SAMIM, Masood, KRULL, Ulrich J
Format: Article
Language:English
Subjects:
Analytical chemistry
Applied sciences
Aptamers, Nucleotide - chemistry
Cell Line, Tumor
Chemistry
Exact sciences and technology
Fluorescence Polarization - methods
Fluorescent Dyes - chemistry
Global environmental pollution
High-Throughput Screening Assays
Humans
Molecular Dynamics Simulation
Nucleic Acids - chemistry
Pollution
Proteins - chemistry
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Summary:The polarization of light emitted by fluorescent probes is an easily accessible physical quantity that is related to a multitude of molecular parameters including conformation, orientation, size and the nanoscale environment conditions, such as dynamic viscosity and temperature. In analytical biochemistry and analytical chemistry applied to biological problems, fluorescence anisotropy is widely used for measuring the folding state of proteins and nucleic acids, and the affinity constant of ligands through titration experiments. The emphasis of this review is on new multi-parameter single-molecule detection schemes and their bioanalytical applications, and on the use of ensemble polarization assays to study binding and conformational dynamics of proteins and aptamers and for high-throughput discovery of small-molecule drugs.
ISSN:0003-2654
1364-5528
DOI:10.1039/b920242k