Fabrication and evaluation of microgrooved polymers as peripheral nerve conduits

Cell alignment plays an important role in the repair of damaged peripheral nerves. The aligned Schwann cells could direct the axonal outgrowth during nerve reconstruction. One way of aligning Schwann cells is to use surface grooves in micrometric dimensions. In this study, microgrooves on chitosan o...

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Published in:Biomedical microdevices 2007-10, Vol.9 (5), p.665-674
Main Authors: Hsu, Shan-hui, Lu, Po Seng, Ni, Hsiao-Chiang, Su, Chien-Hsiang
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Animals, Newborn
Biochemistry
Biocompatible Materials - chemistry
Biodegradation, Environmental
Brain-Derived Neurotrophic Factor - genetics
Brain-Derived Neurotrophic Factor - metabolism
Cell Adhesion
Cell Culture Techniques
Cells, Cultured
Chemical engineering
Chitosan - chemistry
Cytoskeleton - metabolism
Dimethylpolysiloxanes - chemistry
Feasibility Studies
Fluorescent Antibody Technique, Indirect
Gene Expression
Glioma - pathology
Lactic Acid - chemistry
Male
Nerve Growth Factor - genetics
Nerve Growth Factor - metabolism
Peripheral Nerves - cytology
Peripheral Nerves - physiology
Polyesters
Polymers
Polymers - chemistry
Rats
Rats, Sprague-Dawley
S100 Proteins - metabolism
Schwann Cells - cytology
Schwann Cells - physiology
Schwann Cells - transplantation
Sciatic Nerve - cytology
Sciatic Nerve - physiology
Scientific apparatus & instruments
Silicon - chemistry
Time Factors
Vinculin - metabolism
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description Cell alignment plays an important role in the repair of damaged peripheral nerves. The aligned Schwann cells could direct the axonal outgrowth during nerve reconstruction. One way of aligning Schwann cells is to use surface grooves in micrometric dimensions. In this study, microgrooves on chitosan or poly(D,L-lactide) (PLA) were fabricated and the behaviors of Schwann cells and glial cell line C6 on these surfaces were examined. It was found that Schwann cells and C6 cells could be successfully aligned by the microgrooves, and express the genes related to the production of neurotrophic factors. The polymer conduits with microgrooves on the inner surface were implanted in rats to repair the damaged sciatic nerve. The microgrooved conduits were demonstrated to enhance peripheral nerve regeneration as compared to the smooth conduits.
doi_str_mv 10.1007/s10544-007-9068-0
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subjects Actins - metabolism
Animals
Animals, Newborn
Biochemistry
Biocompatible Materials - chemistry
Biodegradation, Environmental
Brain-Derived Neurotrophic Factor - genetics
Brain-Derived Neurotrophic Factor - metabolism
Cell Adhesion
Cell Culture Techniques
Cells, Cultured
Chemical engineering
Chitosan - chemistry
Cytoskeleton - metabolism
Dimethylpolysiloxanes - chemistry
Feasibility Studies
Fluorescent Antibody Technique, Indirect
Gene Expression
Glioma - pathology
Lactic Acid - chemistry
Male
Nerve Growth Factor - genetics
Nerve Growth Factor - metabolism
Peripheral Nerves - cytology
Peripheral Nerves - physiology
Polyesters
Polymers
Polymers - chemistry
Rats
Rats, Sprague-Dawley
S100 Proteins - metabolism
Schwann Cells - cytology
Schwann Cells - physiology
Schwann Cells - transplantation
Sciatic Nerve - cytology
Sciatic Nerve - physiology
Scientific apparatus & instruments
Silicon - chemistry
Time Factors
Vinculin - metabolism
title Fabrication and evaluation of microgrooved polymers as peripheral nerve conduits
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