Effect of low level laser therapy on bronchial hyper-responsiveness

• Published in: Lasers in medical science 2009-07, Vol.24 (4), p.567-576
• Main Authors: Aimbire, Flávio, de Lima, Flávia Mafra, Costa, Maricilia S., Albertini, Regiane, Correa, João Carlos, Iversen, Vegard V., Bjordal, Jan M.
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Bronchial Hyperreactivity - chemically induced; Bronchial Hyperreactivity - physiopathology; Bronchial Hyperreactivity - radiotherapy; Calcium - metabolism; Carbachol - pharmacology; Dentistry; DNA Primers - genetics; Gene Expression - radiation effects; In Vitro Techniques; Inositol 1,4,5-Trisphosphate Receptors - genetics; Inositol 1,4,5-Trisphosphate Receptors - metabolism; Inositol Phosphates - metabolism; Lasers; Low-Level Light Therapy; Macrocyclic Compounds - pharmacology; Male; Medicine; Medicine & Public Health; Muscle Contraction - drug effects; Muscle Contraction - radiation effects; Myocytes, Smooth Muscle - drug effects; Myocytes, Smooth Muscle - physiology; Myocytes, Smooth Muscle - radiation effects; Optical Devices; Optics; Original Article; Oxazoles - pharmacology; Photonics; Quantum Optics; Rats; Rats, Wistar; Respiratory system; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rodents; Therapy; Tumor Necrosis Factor-alpha - pharmacology
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-008-0612-9

The objective of this study was to investigate whether low level laser therapy (LLLT) could reduce bronchial hyper-responsiveness (BHR) induced by tumour necrosis factor-alpha (TNF-α) modulating the metabolism of inositol phosphate (IP) in bronchial smooth muscle cells (BSMCs). The study was on 28 Wistar rats, randomly divided into four groups. Irradiation (1.3 J/cm 2 ) was administered 5 min and 4 h after bronchial smooth muscle (BSM) had been suspended in TNF-α baths, and the contractile response-induced calcium ion (Ca 2+ ) sensitization was measured. The BSMCs were isolated, and the IP accumulation was measured before and after TNF-α immersion in the groups that had been irradiated or not irradiated. BSM segments significantly increased contraction 24 h after TNF-α immersion when exposed to carbachol (CCh) as Ca 2+ , but it was significantly reduced by 64% and 30%, respectively, after laser treatment. The increase in IP accumulation induced by CCh after TNF-α immersion was reduced in the BSMCs by LLLT. The dose of 2.6 J/cm 2 reduced BHR and IP accumulation in the rats’ inflammatory BSMCs.