Identification of a Subspecies-Specific Capsular Antigen from Bacteroides melaninogenicus Subspecies asaccharolyticus by Immunofluorescence and Electron Microscopy

An indirect fluorescent antibody test was developed with the use of hyperimmune rabbit antiserum to a purified capsular polysaccharide of Bacteroides melaninogenicus subspecies asaccharolyticus. All of 23 strains of B. melaninogenicus subspecies asaccharolyticus were fluorescence-positive in this te...

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Bibliographic Details
Published in:The Journal of infectious diseases 1978-12, Vol.138 (6), p.736-741
Main Authors: Mansheim, Bernard J., Solstad, Christina A., Kasper, Dennis L.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigens
Antigens, Bacterial - isolation & purification
Antiserum
Bacteroides
Bacteroides - immunology
Capsules
Cell Membrane - immunology
Electron microscopy
Fluorescence
Fluorescent Antibody Technique
Immune Sera
Lipopolysaccharides - immunology
Microscopy, Electron
Polysaccharides
Prevotella melaninogenica
Prevotella melaninogenica - classification
Prevotella melaninogenica - immunology
Prevotella melaninogenica - ultrastructure
Rabbits
Ruthenium
Ruthenium Red
Scientific Articles
Staining and Labeling - methods
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Description
Summary:An indirect fluorescent antibody test was developed with the use of hyperimmune rabbit antiserum to a purified capsular polysaccharide of Bacteroides melaninogenicus subspecies asaccharolyticus. All of 23 strains of B. melaninogenicus subspecies asaccharolyticus were fluorescence-positive in this test. All II strains of Bacteroides melaninogenicus subspecies intermedius tested and three strains of Bacteroides melaninogenicus subspecies melaninogenicus were fluorescence-negative. Thirty-one strains of other bacterial species were also fluorescence-negative. The indirect fluorescent antibody test demonstrated the presence of a subspecies-specific capsular antigen from B. melaninogenicus subspecies asaccharolyticus. The capsular antigen was further demonstrated by electron microscopy with ruthenium red, a polysaccharide-staining material.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/138.6.736