Loading…

Identification of a Subspecies-Specific Capsular Antigen from Bacteroides melaninogenicus Subspecies asaccharolyticus by Immunofluorescence and Electron Microscopy

An indirect fluorescent antibody test was developed with the use of hyperimmune rabbit antiserum to a purified capsular polysaccharide of Bacteroides melaninogenicus subspecies asaccharolyticus. All of 23 strains of B. melaninogenicus subspecies asaccharolyticus were fluorescence-positive in this te...

Full description

Saved in:

Bibliographic Details
Published in:	The Journal of infectious diseases 1978-12, Vol.138 (6), p.736-741
Main Authors:	Mansheim, Bernard J., Solstad, Christina A., Kasper, Dennis L.
Format:	Article
Language:	English
Subjects:	Animals Antibodies Antigens Antigens, Bacterial - isolation & purification Antiserum Bacteroides Bacteroides - immunology Capsules Cell Membrane - immunology Electron microscopy Fluorescence Fluorescent Antibody Technique Immune Sera Lipopolysaccharides - immunology Microscopy, Electron Polysaccharides Prevotella melaninogenica Prevotella melaninogenica - classification Prevotella melaninogenica - immunology Prevotella melaninogenica - ultrastructure Rabbits Ruthenium Ruthenium Red Scientific Articles Staining and Labeling - methods
Citations:	Items that cite this one
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by	cdi_FETCH-LOGICAL-c267t-bc029ac104b2f00604ee28b629c367f0e98ae9f9ce431912bb69605fa45839aa3
cites
container_end_page	741
container_issue	6
container_start_page	736
container_title	The Journal of infectious diseases
container_volume	138
creator	Mansheim, Bernard J. Solstad, Christina A. Kasper, Dennis L.
description	An indirect fluorescent antibody test was developed with the use of hyperimmune rabbit antiserum to a purified capsular polysaccharide of Bacteroides melaninogenicus subspecies asaccharolyticus. All of 23 strains of B. melaninogenicus subspecies asaccharolyticus were fluorescence-positive in this test. All II strains of Bacteroides melaninogenicus subspecies intermedius tested and three strains of Bacteroides melaninogenicus subspecies melaninogenicus were fluorescence-negative. Thirty-one strains of other bacterial species were also fluorescence-negative. The indirect fluorescent antibody test demonstrated the presence of a subspecies-specific capsular antigen from B. melaninogenicus subspecies asaccharolyticus. The capsular antigen was further demonstrated by electron microscopy with ruthenium red, a polysaccharide-staining material.
doi_str_mv	10.1093/infdis/138.6.736
format	article
fullrecord	<record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_74361919</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30109035</jstor_id><sourcerecordid>30109035</sourcerecordid><originalsourceid>FETCH-LOGICAL-c267t-bc029ac104b2f00604ee28b629c367f0e98ae9f9ce431912bb69605fa45839aa3</originalsourceid><addsrcrecordid>eNpNkU9v1DAQxS1EBUvhjoSQfOKW7ThOnPhYVv2zaisOC1LFxXKcMbgkcWonEvt5-KL1NtWK0xx-857ezCPkI4M1A8nP3GBbF88Yr9diXXHxiqxYyatMCMZfkxVAnmeslvIteRfjAwAUXFRvyEldABcr8m_b4jA564yenB-ot1TT3dzEEY3DmO0OM1G60WOcOx3oeVr_hQO1wff0qzYTBu9ajLTHTg9u8Ak6M8f_XKiO2pjfOvhuPz2zZk-3fT8P3nazDxgNDgapHlp60aGZQkpy50zw0fhx_56cWN1F_PAyT8mPy4vvm-vs9tvVdnN-m5lcVFPWGMilNgyKJrcAAgrEvG5ELk062QLKWqO00mDBmWR50wgpoLS6KGsutean5MviOwb_OGOcVO9Ssi6dhX6OqkqvS0KZFmFZPCSMAa0ag-t12CsG6tCKWlpRqRUlVGolST69eM9Nj-1R8FxDop8X-hAnH46QQzIDXiaeLdzFCf8euQ5_lKh4Varr-5_q6vK-KOTNTt3xJw5Qp1E</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74361919</pqid></control><display><type>article</type><title>Identification of a Subspecies-Specific Capsular Antigen from Bacteroides melaninogenicus Subspecies asaccharolyticus by Immunofluorescence and Electron Microscopy</title><source>JSTOR Archival Journals and Primary Sources Collection</source><source>Oxford University Press:Jisc Collections:Oxford Journal Archive: Access period 2024-2025</source><creator>Mansheim, Bernard J. ; Solstad, Christina A. ; Kasper, Dennis L.</creator><creatorcontrib>Mansheim, Bernard J. ; Solstad, Christina A. ; Kasper, Dennis L.</creatorcontrib><description>An indirect fluorescent antibody test was developed with the use of hyperimmune rabbit antiserum to a purified capsular polysaccharide of Bacteroides melaninogenicus subspecies asaccharolyticus. All of 23 strains of B. melaninogenicus subspecies asaccharolyticus were fluorescence-positive in this test. All II strains of Bacteroides melaninogenicus subspecies intermedius tested and three strains of Bacteroides melaninogenicus subspecies melaninogenicus were fluorescence-negative. Thirty-one strains of other bacterial species were also fluorescence-negative. The indirect fluorescent antibody test demonstrated the presence of a subspecies-specific capsular antigen from B. melaninogenicus subspecies asaccharolyticus. The capsular antigen was further demonstrated by electron microscopy with ruthenium red, a polysaccharide-staining material.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/138.6.736</identifier><identifier>PMID: 84036</identifier><language>eng</language><publisher>United States: The University of Chicago Press</publisher><subject>Animals ; Antibodies ; Antigens ; Antigens, Bacterial - isolation & purification ; Antiserum ; Bacteroides ; Bacteroides - immunology ; Capsules ; Cell Membrane - immunology ; Electron microscopy ; Fluorescence ; Fluorescent Antibody Technique ; Immune Sera ; Lipopolysaccharides - immunology ; Microscopy, Electron ; Polysaccharides ; Prevotella melaninogenica ; Prevotella melaninogenica - classification ; Prevotella melaninogenica - immunology ; Prevotella melaninogenica - ultrastructure ; Rabbits ; Ruthenium ; Ruthenium Red ; Scientific Articles ; Staining and Labeling - methods</subject><ispartof>The Journal of infectious diseases, 1978-12, Vol.138 (6), p.736-741</ispartof><rights>Copyright 1978 The University of Chicago</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c267t-bc029ac104b2f00604ee28b629c367f0e98ae9f9ce431912bb69605fa45839aa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30109035$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30109035$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/84036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mansheim, Bernard J.</creatorcontrib><creatorcontrib>Solstad, Christina A.</creatorcontrib><creatorcontrib>Kasper, Dennis L.</creatorcontrib><title>Identification of a Subspecies-Specific Capsular Antigen from Bacteroides melaninogenicus Subspecies asaccharolyticus by Immunofluorescence and Electron Microscopy</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>An indirect fluorescent antibody test was developed with the use of hyperimmune rabbit antiserum to a purified capsular polysaccharide of Bacteroides melaninogenicus subspecies asaccharolyticus. All of 23 strains of B. melaninogenicus subspecies asaccharolyticus were fluorescence-positive in this test. All II strains of Bacteroides melaninogenicus subspecies intermedius tested and three strains of Bacteroides melaninogenicus subspecies melaninogenicus were fluorescence-negative. Thirty-one strains of other bacterial species were also fluorescence-negative. The indirect fluorescent antibody test demonstrated the presence of a subspecies-specific capsular antigen from B. melaninogenicus subspecies asaccharolyticus. The capsular antigen was further demonstrated by electron microscopy with ruthenium red, a polysaccharide-staining material.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Antigens, Bacterial - isolation & purification</subject><subject>Antiserum</subject><subject>Bacteroides</subject><subject>Bacteroides - immunology</subject><subject>Capsules</subject><subject>Cell Membrane - immunology</subject><subject>Electron microscopy</subject><subject>Fluorescence</subject><subject>Fluorescent Antibody Technique</subject><subject>Immune Sera</subject><subject>Lipopolysaccharides - immunology</subject><subject>Microscopy, Electron</subject><subject>Polysaccharides</subject><subject>Prevotella melaninogenica</subject><subject>Prevotella melaninogenica - classification</subject><subject>Prevotella melaninogenica - immunology</subject><subject>Prevotella melaninogenica - ultrastructure</subject><subject>Rabbits</subject><subject>Ruthenium</subject><subject>Ruthenium Red</subject><subject>Scientific Articles</subject><subject>Staining and Labeling - methods</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><recordid>eNpNkU9v1DAQxS1EBUvhjoSQfOKW7ThOnPhYVv2zaisOC1LFxXKcMbgkcWonEvt5-KL1NtWK0xx-857ezCPkI4M1A8nP3GBbF88Yr9diXXHxiqxYyatMCMZfkxVAnmeslvIteRfjAwAUXFRvyEldABcr8m_b4jA564yenB-ot1TT3dzEEY3DmO0OM1G60WOcOx3oeVr_hQO1wff0qzYTBu9ajLTHTg9u8Ak6M8f_XKiO2pjfOvhuPz2zZk-3fT8P3nazDxgNDgapHlp60aGZQkpy50zw0fhx_56cWN1F_PAyT8mPy4vvm-vs9tvVdnN-m5lcVFPWGMilNgyKJrcAAgrEvG5ELk062QLKWqO00mDBmWR50wgpoLS6KGsutean5MviOwb_OGOcVO9Ssi6dhX6OqkqvS0KZFmFZPCSMAa0ag-t12CsG6tCKWlpRqRUlVGolST69eM9Nj-1R8FxDop8X-hAnH46QQzIDXiaeLdzFCf8euQ5_lKh4Varr-5_q6vK-KOTNTt3xJw5Qp1E</recordid><startdate>197812</startdate><enddate>197812</enddate><creator>Mansheim, Bernard J.</creator><creator>Solstad, Christina A.</creator><creator>Kasper, Dennis L.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197812</creationdate><title>Identification of a Subspecies-Specific Capsular Antigen from Bacteroides melaninogenicus Subspecies asaccharolyticus by Immunofluorescence and Electron Microscopy</title><author>Mansheim, Bernard J. ; Solstad, Christina A. ; Kasper, Dennis L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c267t-bc029ac104b2f00604ee28b629c367f0e98ae9f9ce431912bb69605fa45839aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Antigens, Bacterial - isolation & purification</topic><topic>Antiserum</topic><topic>Bacteroides</topic><topic>Bacteroides - immunology</topic><topic>Capsules</topic><topic>Cell Membrane - immunology</topic><topic>Electron microscopy</topic><topic>Fluorescence</topic><topic>Fluorescent Antibody Technique</topic><topic>Immune Sera</topic><topic>Lipopolysaccharides - immunology</topic><topic>Microscopy, Electron</topic><topic>Polysaccharides</topic><topic>Prevotella melaninogenica</topic><topic>Prevotella melaninogenica - classification</topic><topic>Prevotella melaninogenica - immunology</topic><topic>Prevotella melaninogenica - ultrastructure</topic><topic>Rabbits</topic><topic>Ruthenium</topic><topic>Ruthenium Red</topic><topic>Scientific Articles</topic><topic>Staining and Labeling - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mansheim, Bernard J.</creatorcontrib><creatorcontrib>Solstad, Christina A.</creatorcontrib><creatorcontrib>Kasper, Dennis L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mansheim, Bernard J.</au><au>Solstad, Christina A.</au><au>Kasper, Dennis L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a Subspecies-Specific Capsular Antigen from Bacteroides melaninogenicus Subspecies asaccharolyticus by Immunofluorescence and Electron Microscopy</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1978-12</date><risdate>1978</risdate><volume>138</volume><issue>6</issue><spage>736</spage><epage>741</epage><pages>736-741</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><abstract>An indirect fluorescent antibody test was developed with the use of hyperimmune rabbit antiserum to a purified capsular polysaccharide of Bacteroides melaninogenicus subspecies asaccharolyticus. All of 23 strains of B. melaninogenicus subspecies asaccharolyticus were fluorescence-positive in this test. All II strains of Bacteroides melaninogenicus subspecies intermedius tested and three strains of Bacteroides melaninogenicus subspecies melaninogenicus were fluorescence-negative. Thirty-one strains of other bacterial species were also fluorescence-negative. The indirect fluorescent antibody test demonstrated the presence of a subspecies-specific capsular antigen from B. melaninogenicus subspecies asaccharolyticus. The capsular antigen was further demonstrated by electron microscopy with ruthenium red, a polysaccharide-staining material.</abstract><cop>United States</cop><pub>The University of Chicago Press</pub><pmid>84036</pmid><doi>10.1093/infdis/138.6.736</doi><tpages>6</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0022-1899
ispartof	The Journal of infectious diseases, 1978-12, Vol.138 (6), p.736-741
issn	0022-1899 1537-6613
language	eng
recordid	cdi_proquest_miscellaneous_74361919
source	JSTOR Archival Journals and Primary Sources Collection; Oxford University Press:Jisc Collections:Oxford Journal Archive: Access period 2024-2025
subjects	Animals Antibodies Antigens Antigens, Bacterial - isolation & purification Antiserum Bacteroides Bacteroides - immunology Capsules Cell Membrane - immunology Electron microscopy Fluorescence Fluorescent Antibody Technique Immune Sera Lipopolysaccharides - immunology Microscopy, Electron Polysaccharides Prevotella melaninogenica Prevotella melaninogenica - classification Prevotella melaninogenica - immunology Prevotella melaninogenica - ultrastructure Rabbits Ruthenium Ruthenium Red Scientific Articles Staining and Labeling - methods
title	Identification of a Subspecies-Specific Capsular Antigen from Bacteroides melaninogenicus Subspecies asaccharolyticus by Immunofluorescence and Electron Microscopy
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T08%3A08%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20a%20Subspecies-Specific%20Capsular%20Antigen%20from%20Bacteroides%20melaninogenicus%20Subspecies%20asaccharolyticus%20by%20Immunofluorescence%20and%20Electron%20Microscopy&rft.jtitle=The%20Journal%20of%20infectious%20diseases&rft.au=Mansheim,%20Bernard%20J.&rft.date=1978-12&rft.volume=138&rft.issue=6&rft.spage=736&rft.epage=741&rft.pages=736-741&rft.issn=0022-1899&rft.eissn=1537-6613&rft_id=info:doi/10.1093/infdis/138.6.736&rft_dat=%3Cjstor_proqu%3E30109035%3C/jstor_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c267t-bc029ac104b2f00604ee28b629c367f0e98ae9f9ce431912bb69605fa45839aa3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=74361919&rft_id=info:pmid/84036&rft_jstor_id=30109035&rfr_iscdi=true