Semiconductor Nanocrystals as Fluorescent Biological Labels

Semiconductor nanocrystals were prepared for use as fluorescent probes in biological staining and diagnostics. Compared with conventional fluorophores, the nanocrystals have a narrow, tunable, symmetric emission spectrum and are photochemically stable. The advantages of the broad, continuous excitat...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1998-09, Vol.281 (5385), p.2013-2016
Main Authors: Bruchez, Marcel, Moronne, Mario, Gin, Peter, Weiss, Shimon, Alivisatos, A. Paul
Format: Article
Language:English
Subjects:
3T3 Cells
Actins
Animals
Biological and medical sciences
Biology
Biotinylation
Cadmium Compounds
Cell Nucleus - ultrastructure
Chemistry
Crystallization
Cytometry
Cytoskeleton - ultrastructure
Diverse techniques
Dyes
Emission spectra
Fluorescence
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
Materials science
Mice
Microscopy, Confocal
Microscopy, Fluorescence
Molecular and cellular biology
Molecular Probe Techniques
Molecules
Nanocrystals
Organic Chemistry
Rodents
Scientific Concepts
Selenium Compounds
Semiconductors
Silicon Dioxide
Solubility
Spectrometry, Fluorescence
Staining and Labeling - methods
Sulfides
Water
Wavelengths
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Description
Summary:Semiconductor nanocrystals were prepared for use as fluorescent probes in biological staining and diagnostics. Compared with conventional fluorophores, the nanocrystals have a narrow, tunable, symmetric emission spectrum and are photochemically stable. The advantages of the broad, continuous excitation spectrum were demonstrated in a dual-emission, single-excitation labeling experiment on mouse fibroblasts. These nanocrystal probes are thus complementary and in some cases may be superior to existing fluorophores.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.281.5385.2013