A family of mammalian Na + -dependent L -ascorbic acid transporters

• Published in: Nature (London) 1999-05, Vol.399 (6731), p.70-75
• Main Authors: Hediger, Matthias A, Tsukaguchi, Hiroyasu, Tokui, Taro, Mackenzie, Bryan, Berger, Urs V, Chen, Xing-Zhen, Wang, Yangxi, Brubaker, Richard F
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Ascorbic Acid - metabolism; Biological and medical sciences; Biological Transport; Cloning, Molecular; DNA, Complementary; Electrochemistry; Enzymes; Fundamental and applied biological sciences. Psychology; Genes. Genome; Humanities and Social Sciences; letter; Mammals; Metabolism; Metal ions; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; multidisciplinary; Organic Anion Transporters, Sodium-Dependent; Proteins; Proteins - genetics; Proteins - isolation & purification; Proteins - metabolism; Rabbits; Rats; Science; Science (multidisciplinary); Sequence Homology, Amino Acid; Sodium - metabolism; Sodium-Coupled Vitamin C Transporters; Symporters; Tissue Distribution; Vitamin C; Xenopus
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/19986

Vitamin C (L-ascorbic acid) is essential for many enzymatic reactions, in which it serves to maintain prosthetic metal ions in their reduced forms (for example, Fe2+, Cu+),, and for scavenging free radicals in order to protect tissues from oxidative damage. The facilitative sugar transporters of the GLUT type can transport the oxidized form of the vitamin, dehydroascorbic acid, but these transporters are unlikely to allow significant physiological amounts of vitamin C to be taken up in the presence of normal glucose concentrations, because the vitamin is present in plasma essentially only in its reduced form. Here we describe the isolation of two L-ascorbic acid transporters, SVCT1 and SVCT2, from rat complementary DNA libraries, as the first step in investigating the importance of L-ascorbic acid transport in regulating the supply and metabolism of vitamin C. We find that SVCT1 and SVCT2 each mediate concentrative, high-affinity L-ascorbic acid transport that is stereospecific and is driven by the Na+ electrochemical gradient. Despite their close sequence homology and similar functions, the two isoforms of the transporter are discretely distributed: SVCT1 is mainly confined to epithelial systems (intestine, kidney, liver), whereas SVCT2 serves a host of metabolically active cells and specialized tissues in the brain, eye and other organs.