Gene Targeting by Homologous Recombination in Drosophila

Drosophila offers many advantages as an experimental organism. However, in comparison with yeast and mouse, two other widely used eukaryotic model systems, Drosophila suffers from an inability to perform homologous recombination between introduced DNA and the corresponding chromosomal loci. The abil...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2000-06, Vol.288 (5473), p.2013-2018
Main Authors: Rong, Yikang S., Golic, Kent G.
Format: Article
Language:English
Subjects:
Alleles
Animals
Biological and medical sciences
Biotechnology
Blotting, Southern
Cloning, Molecular
Crystals
Deoxyribonucleases, Type II Site-Specific - genetics
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA
DNA Damage
DNA Nucleotidyltransferases - metabolism
DNA Repair
Donors
Drosophila
Drosophila melanogaster - genetics
Female
Fundamental and applied biological sciences. Psychology
Gene Targeting
Gene therapy
Genes
Genes, Insect
Genetic engineering
Genetic loci
Genetic technics
Genetics
Germ cells
homologous recombination
In Situ Hybridization
Insects
Male
Methods. Procedures. Technologies
Mutagenesis
Mutation
Point Mutation
Poisson ratio
Recombination, Genetic
Saccharomyces cerevisiae Proteins
Transgenes
Transgenic animals
Transgenic animals and transgenic plants
Transposons
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Drosophila offers many advantages as an experimental organism. However, in comparison with yeast and mouse, two other widely used eukaryotic model systems, Drosophila suffers from an inability to perform homologous recombination between introduced DNA and the corresponding chromosomal loci. The ability to specifically modify the genomes of yeast and mouse provides a quick and easy way to generate or rescue mutations in genes for which a DNA clone or sequence is available. A method is described that enables analogous manipulations of the Drosophila genome. This technique may also be applicable to other organisms for which gene-targeting procedures do not yet exist.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.288.5473.2013