Myosin-V is a processive actin-based motor

Class-V myosins, one of 15 known classes of actin-based molecular motors, have been implicated in several forms of organelle transport perhaps working with microtubule-based motors such as kinesin,. Such movements may require a motor with mechanochemical properties distinct from those of myosin-II,...

Full description

Saved in:
Bibliographic Details
Published in:Nature (London) 1999-08, Vol.400 (6744), p.590-593
Main Authors: Mehta, Amit D, Cheney, Richard E, Rock, Ronald S, Rief, Matthias, Spudich, James A, Mooseker, Mark S
Format: Article
Language:English
Subjects:
Actins - physiology
Adenosine Triphosphate - metabolism
Analytical, structural and metabolic biochemistry
Animals
Biochemistry
Biological and medical sciences
Calmodulin-Binding Proteins - physiology
Catalysis
Chickens
Contractile proteins
Fundamental and applied biological sciences. Psychology
Holoproteins
Humanities and Social Sciences
In Vitro Techniques
letter
Molecular biology
Molecular Motor Proteins - physiology
Movement
multidisciplinary
Myosin Light Chains - physiology
Myosin Type V
Nerve Tissue Proteins - physiology
Optics and Photonics
Proteins
Science
Science (multidisciplinary)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Class-V myosins, one of 15 known classes of actin-based molecular motors, have been implicated in several forms of organelle transport perhaps working with microtubule-based motors such as kinesin,. Such movements may require a motor with mechanochemical properties distinct from those of myosin-II, which operates in large ensembles to drive high-speed motility as in muscle contraction. Based on its function and biochemistry, it has been suggested that myosin-V may be a processive motor, like kinesin,. Processivity means that the motor undergoes multiple catalytic cycles and coupled mechanical advances for each diffusional encounter with its track. This allows single motors to support movement of an organelle along its track. Here we provide direct evidence that myosin-V is indeed a processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament.
ISSN:0028-0836
1476-4687
DOI:10.1038/23072