Plant paralog to viral movement protein that potentiates transport of mRNA into the phloem

CmPP16 from Cucurbita maxima was cloned and the protein was shown to possess properties similar to those of viral movement proteins. CmPP16 messenger RNA (mRNA) is present in phloem tissue, whereas protein appears confined to sieve elements (SE). Microinjection and grafting studies revealed that CmP...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1999, Vol.283 (5398), p.94-98
Main Authors: Xoconostle-Cazares, B, Xiang, Y, Ruiz-Medrano, R, Wang, H.L, Monzer, J, Yoo, B.C, McFarland, K.C, Franceschi, V.R, Lucas, W.J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antiserum
Biological and medical sciences
Biological Transport
Botany
cloning
Cloning, Molecular
Complementary DNA
Cucumbers
Cucumis sativus
Cucurbita maxima
Cucurbitaceae - genetics
Cucurbitaceae - metabolism
DNA
Fundamental and applied biological sciences. Psychology
Messenger RNA
Microinjections
Molecular Sequence Data
Observation
Phloem
Plant Leaves - metabolism
Plant physiology and development
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - metabolism
Plant Stems - metabolism
Plant Viral Movement Proteins
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA, Antisense - metabolism
RNA, Messenger - metabolism
RNA, Plant - metabolism
Scions
Signal Transduction
Space life sciences
translocation (plant physiology)
Viral Proteins - chemistry
Viral Proteins - metabolism
Water and solutes. Absorption, translocation and permeability
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Summary:CmPP16 from Cucurbita maxima was cloned and the protein was shown to possess properties similar to those of viral movement proteins. CmPP16 messenger RNA (mRNA) is present in phloem tissue, whereas protein appears confined to sieve elements (SE). Microinjection and grafting studies revealed that CmPP16 moves from cell to cell, mediates the transport of sense and antisense RNA, and moves together with its mRNA into the SE of scion tissue. CmPP16 possesses the characteristics that are likely required to mediate RNA delivery into the long-distance translocation stream. Thus, RNA may move within the phloem as a component of a plant information superhighway.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.283.5398.94