Arabidopsis NPH1: a flavoprotein with the properties of a photoreceptor for phototropism

The NPH1 gene of Arabidopsis thaliana encodes a 120-kilodalton serine-threonine protein kinase hypothesized to function as a photoreceptor for phototropism. When expressed in insect cells, the NPH1 protein is phosphorylated in response to blue light irradiation. The biochemical and photochemical pro...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1998-11, Vol.282 (5394), p.1698-1701
Main Authors: Christie, J.M. (Carnegie Institution of Washington, Stanford, CA.), Reymond, P, Powell, G.K, Bernasconi, P, Raibekas, A.A, Liscum, E, Briggs, W.R
Format: Article
Language:English
Subjects:
Absorption spectra
Animals
ARABIDOPSIS
Arabidopsis - genetics
Arabidopsis - physiology
Arabidopsis Proteins
Biological and medical sciences
Botany
Cell Line
Cell membranes
Chromophores
Classical genetics, quantitative genetics, hybrids
Coding
Cryptochromes
Drosophila Proteins
Environmental aspects
EXPRESION GENICA
EXPRESSION DES GENES
Eye Proteins
Feedback (Response)
Flavin Mononucleotide - metabolism
Flavoproteins - physiology
Flowers & plants
Fluorescence
FOTORECEPTORES
Fractions
Fundamental and applied biological sciences. Psychology
GENE
GENE EXPRESSION
GENES
Genes, Plant
Genetics of eukaryotes. Biological and molecular evolution
Insect proteins
Insect vectors
Insects
Light
Line spectra
Mutation
Phosphoproteins - genetics
Phosphoproteins - metabolism
Phosphorylation
PHOTORECEPTEUR
Photoreceptor Cells, Invertebrate
PHOTORECEPTORS
Phototropism
Protein kinases
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Proteins
Pteridophyta, spermatophyta
Receptors, G-Protein-Coupled
Recombinant Proteins - metabolism
Space life sciences
Spectrometry, Fluorescence
Spodoptera
Transfection
TROPISME
TROPISMO
TROPISMS
Vegetals
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Description
Summary:The NPH1 gene of Arabidopsis thaliana encodes a 120-kilodalton serine-threonine protein kinase hypothesized to function as a photoreceptor for phototropism. When expressed in insect cells, the NPH1 protein is phosphorylated in response to blue light irradiation. The biochemical and photochemical properties of the photosensitive protein reflect those of the native protein in microsomal membranes. Recombinant NPH1 noncovalently binds flavin mononucleotide a likely chromophore for light-dependent autophosphorylation. The fluorescence excitation spectrum of the recombinant protein is similar to the action spectrum for phototropism, consistent with the conclusion that NPH1 is an autophosphorylating flavoprotein photoreceptor mediating phototropic responses in higher plants
ISSN:0036-8075
1095-9203
DOI:10.1126/science.282.5394.1698