The Protein Kinase p90 Rsk as an Essential Mediator of Cytostatic Factor Activity

Persistent activation of p42 mitogen-activated protein kinase (p42 MAPK) during mitosis induces a "cytostatic factor" arrest, the arrest responsible for preventing the parthenogenetic activation of unfertilized eggs. The protein kinase p90 Rsk is a substrate of p42 MAPK; thus, the role of...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1999-11, Vol.286 (5443), p.1362-1365
Main Authors: Bhatt, Ramesh R., Ferrell, James E.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
CDC2 Protein Kinase - metabolism
Cell Extracts
Cell physiology
Cellular biology
Chromatin
Cytosine
Eggs
Embryos
Enzyme Activation
Fundamental and applied biological sciences. Psychology
Histones
Immunoblotting
MAP Kinase Kinase 1
MAP Kinase Signaling System
Metaphase
Microscopy
Mitogen-Activated Protein Kinase 1 - metabolism
Mitogen-Activated Protein Kinase Kinases - metabolism
Mitosis
Molecular and cellular biology
Molecular Sequence Data
Nuclear membrane
Oocytes
Ovum - cytology
Ovum - enzymology
Phosphorylation
Protein kinases
Protein-Serine-Threonine Kinases
Proteins
Proto-Oncogene Proteins c-mos - metabolism
Proto-Oncogene Proteins c-mos - pharmacology
Ribosomal Protein S6 Kinases - metabolism
Signal transduction
Vertebrates
Xenopus
Xenopus laevis
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Summary:Persistent activation of p42 mitogen-activated protein kinase (p42 MAPK) during mitosis induces a "cytostatic factor" arrest, the arrest responsible for preventing the parthenogenetic activation of unfertilized eggs. The protein kinase p90 Rsk is a substrate of p42 MAPK; thus, the role of p90 Rsk in p42 MAPK-induced mitotic arrest was examined. Xenopus laevis egg extracts immunodepleted of Rsk lost their capacity to undergo mitotic arrest in response to activation of the Mos-MEK-1-p42 MAPK cascade of protein kinases. Replenishing Rsk-depleted extracts with catalytically competent Rsk protein restored the ability of the extracts to undergo mitotic arrest. Rsk appears to be essential for cytostatic factor arrest.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.286.5443.1362