Pex16p homolog SSE1 and storage organelle formation in Arabidopsis seeds

• Published in: Science (American Association for the Advancement of Science) 1999-04, Vol.284 (5412), p.328-330
• Main Authors: Lin, Y, Nguyen, L.V, Rachubinski, R.A, Goodman, H.M
• Format: Article
• Language: English
• Subjects: Alleles; Amino Acid Sequence; amino acid sequences; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis - ultrastructure; Arabidopsis Proteins; biochemical pathways; Biological and medical sciences; Cell organelles; Classical genetics, quantitative genetics, hybrids; Complementary DNA; Cotyledons; Fundamental and applied biological sciences. Psychology; Fungal Proteins; Gene Expression; Genetic Complementation Test; Genetics of eukaryotes. Biological and molecular evolution; Genotypes; Lipid bodies; Lipids; Membrane Proteins - chemistry; Membrane Proteins - genetics; Microbodies - metabolism; Microbodies - ultrastructure; Microscopy, Electron; Molecular Sequence Data; mutants; Mutation; Oils & fats; Organelles; Organelles - metabolism; Organelles - ultrastructure; Peroxins; Peroxisomes; Phenotype; Plant Oils - metabolism; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Proteins - physiology; Plants; Proteins; Pteridophyta, spermatophyta; Reverse Transcriptase Polymerase Chain Reaction; Saccharomycetales - chemistry; Saccharomycetales - genetics; Saccharomycetales - metabolism; Seeds; Seeds - metabolism; Seeds - ultrastructure; starch; Starch - metabolism; starch accumulation; Starches; Storage; Vegetals
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.284.5412.328

Mature Arabidopsis seeds are enriched in storage proteins and lipids, but lack starch. In the shrunken seed 1 (sse1) mutant, however, starch is favored over proteins and lipids as the major storage compound. SSE1 has 26 percent identity with Pex16p in Yarrowia lipolytica and complements pex16 mutants defective in the formation of peroxisomes and the transportation of plasma membrane- and cell wall-associated proteins. In Arabidopsis maturing seeds, SSE1 is required for protein and oil body biogenesis, both of which are endoplasmic reticulum-dependent. Starch accumulation in sse1 suggests that starch formation is a default storage deposition pathway.