DETERMINATION OF UBIQUINONE IN SERUM AND LIVER BY HIGH-SPEED LIQUID CHROMATOGRAPHY

Two kinds of high-speed liquid chromatographic methods using either ultraviolet or a fluorometric detector (HSLC-UV and HSLC-fluorometric methods) are described for the determination of ubiquinones (UQ). HSLC-UV method: Serum or liver was saponified with methanolic alkaline solution containing pyrog...

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Bibliographic Details
Published in:Journal of Nutritional Science and Vitaminology 1978, Vol.24(6), pp.555-567
Main Authors: ABE, Kouichi, ISHIBASHI, Kyoko, OHMAE, Masahiko, KAWABE, Kiyoshi, KATSUI, Goichiro
Format: Article
Language:English
Subjects:
Animals
Chromatography, High Pressure Liquid - methods
Fluorometry
Humans
Liver - analysis
Mice
Ubiquinone - analysis
Ubiquinone - blood
Ultraviolet Rays
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Summary:Two kinds of high-speed liquid chromatographic methods using either ultraviolet or a fluorometric detector (HSLC-UV and HSLC-fluorometric methods) are described for the determination of ubiquinones (UQ). HSLC-UV method: Serum or liver was saponified with methanolic alkaline solution containing pyrogallol as an antioxidant and 2, 3, 6trimethyl-5-nonaprenyl-l, 4-benzoquinone as an internal standard and then extracted with n-hexane. The extract was evaporated and then dissolved in dioxane. The resulting solution was applied to a HSLC system using a Permaphase ODS column, ethanol-water (80:20) as a mobile phase and a high sensitivity UV detector (275 nm). HSLC-fluorometric method: Serum was deproteinized with ethanol and then extracted with n-hexane. The resulting extract was reacted with alkaline ethylcyanoacetate (ECA) reagent to give fluorescence. The reacted solution was applied to a HSLC system using a Permaphase ODS column, ethanol-water (75:25) as a mobile phase and a spectrofluorometer (Ex. 430 nm, Em. 530 nm). Neither of the proposed HSLC methods were affected by other fat-soluble substances and seemed to be more simple, sensitive and specific than other conventional determination methods.
ISSN:0301-4800
1881-7742
DOI:10.3177/jnsv.24.555