Radioimmunoassay of the Insulin Receptor: A New Probe of Receptor Structure and Function

A sensitive and specific radioimmunoassay for the insulin receptor has been developed employing receptor autoantibodies from the serum of a patient with insulin-resistant diabetes. The assay detects insulin binding sites at concentrations as low as 0.1 nanomolar; distinguishes between receptors orig...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1979-02, Vol.203 (4380), p.544-547
Main Authors: Harrison, Len C., Flier, Jeffrey, Itin, Ahuva, Kahn, C. Ronald, Roth, Jesse
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigen-Antibody Reactions
Binding Sites
Binding Sites, Antibody
Diabetes
Epitopes
Female
Humans
Immunoassay
Insulin
Liver - analysis
Lymphocytes
Lymphocytes - analysis
Mice
Molecules
Placenta - analysis
Pregnancy
Radioimmunoassay
Radioimmunoassay - methods
Receptor, Insulin - analysis
Receptor, Insulin - immunology
Receptors
Sodium
Solubility
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Summary:A sensitive and specific radioimmunoassay for the insulin receptor has been developed employing receptor autoantibodies from the serum of a patient with insulin-resistant diabetes. The assay detects insulin binding sites at concentrations as low as 0.1 nanomolar; distinguishes between receptors originating from human placental membranes, human lymphoblastoid cells, and mouse liver membranes; and measures the receptor independently of its binding function. Down-regulation, or loss of binding after exposure to insulin, is associated with loss of immunoreactive receptor.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.83675