CELL SURFACE COAT OF HAMSTER FIBROBLASTS

The UHF fraction from NIL 8 hamster embryo fibroblasts contains the LETS protein and several other major proteins. It exhibits three enzymatic activities in significant amounts: 5'-nucleotidase, alkaline phosphatase, and galactosyl transferase. The latter two appear to be different from the mem...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 1978-06, Vol.312 (1), p.221-239
Main Authors: Graham, John M., Hynes, Richard O., Rowlatt, Charles, Sandall, Jane K.
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Animals
Cell Membrane - metabolism
Cell Transformation, Neoplastic
Cells, Cultured
Cricetinae
Fibroblasts - metabolism
Fibronectins - metabolism
Galactosyltransferases - metabolism
Membrane Proteins - metabolism
Microscopy, Electron
Molecular Weight
Nucleotidases - metabolism
Ruthenium Red
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Summary:The UHF fraction from NIL 8 hamster embryo fibroblasts contains the LETS protein and several other major proteins. It exhibits three enzymatic activities in significant amounts: 5'-nucleotidase, alkaline phosphatase, and galactosyl transferase. The latter two appear to be different from the membrane-bound enzymes. This fraction is heavily stained with ruthenium red, a dye specific for the cell coat in intact cells. A comparable fraction from hamster sarcoma virus-transformed cells exhibits a similar overall protein composition but lacks at least three major proteins, including the LETS protein. Compared to NIL 8 cells, the distribution of alkaline phosphatase in fractions from these cells is different, and the level of galactosyl transferase in the UHF is much reduced.
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1978.tb16805.x