Hydrolysis and Synthesis of Wax Esters by Different Systems of Carp Hepatopancreas Preparation

It was found that the supernatant of hepatopancreas homogenate of the carp, Cyprinus carpio, contained enzymes involved in the hydrolysis and esterification of wax esters. The enzymes were salted out by 30% saturation with ammonium sulfate. This fraction also contained the highest activities of lipa...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1979-01, Vol.85 (1), p.1-6
Main Authors: KAYAMA, Mitsu, MANKURA, Mitsumasa, IKEDA, Yasuyuki
Format: Article
Language:English
Subjects:
Adipose Tissue - enzymology
Animals
Carps
Esterases - metabolism
Esters
Female
Hydrolysis
Intestines - enzymology
Kidney - enzymology
Lipase - metabolism
Liver - metabolism
Ovary - enzymology
Pancreas - metabolism
Waxes - metabolism
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Summary:It was found that the supernatant of hepatopancreas homogenate of the carp, Cyprinus carpio, contained enzymes involved in the hydrolysis and esterification of wax esters. The enzymes were salted out by 30% saturation with ammonium sulfate. This fraction also contained the highest activities of lipase and esterase. The enzyme for the hydrolysis of wax esters, together with lipase and esterase, was eluted in tube numbers 20 to 22 (void volume) from a Sephadex G-200 column. On the other hand, the enzyme for the synthesis of wax esters was eluted in tube numbers 33 to 35. It thus appears that the two activities belong to different enzyme proteins. The synthetic enzyme was activated by the addition of ATP, CoA, and NADH.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a132298