The configuration and location of the ribosidic linkage in the prosthetic group of citrate lyase (Klebsiella aerogenes)

The structure of the prosthetic group of citrate lyase (Klebsiella aerogenes) was studied by nuclear magnetic resonance and mass spectrometry. The spectra at 360 MHz of the nucleoside moiety (2'-ribosyladenosine) show the absence of 2'-hydroxyl proton, thus confirming the 2' position...

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Bibliographic Details
Published in:The Journal of biological chemistry 1979-02, Vol.254 (4), p.1000-1002
Main Authors: Oppenheimer, N J, Singh, M, Sweeley, C C, Sung, S J, Srere, P A
Format: Article
Language:English
Subjects:
Adenosine
ATP Citrate (pro-S)-Lyase
Glycosides - analysis
Klebsiella pneumoniae - enzymology
Magnetic Resonance Spectroscopy
Mass Spectrometry
Molecular Conformation
Ribose - analysis
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Summary:The structure of the prosthetic group of citrate lyase (Klebsiella aerogenes) was studied by nuclear magnetic resonance and mass spectrometry. The spectra at 360 MHz of the nucleoside moiety (2'-ribosyladenosine) show the absence of 2'-hydroxyl proton, thus confirming the 2' position as the site of attachment of the second ribose moiety to the dephospho-CoA. This glycosidic linkage is found to be alpha(1" leads to 2') and is identical to that of poly(ADP-ribose). Studies of permethylation products by mass spectrometry support the above conclusion regarding the location of the ribosidic linkage.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)34155-8