Physicochemical Properties of Pyocin F1

The physicochemical properties of pyocin F1 were studied. Pyocin F1 consists of flexuous rod-like particles homogeneous in size. Each particle was composed of rod and fiber parts. The rod part was 105·5 ±9·5 nm long and 10·0± 1·4 nm wide, and showed regular striations amounting to 23 layers. The fib...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1979-01, Vol.85 (1), p.21-28
Main Authors: KURODA, Kazufumi, KAGEYAMA, Makoto, MAEDA, Tadakazu, FUJIME, Satoru
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Bacteriocins - isolation & purification
Microscopy, Electron
Molecular Weight
Protein Conformation
Pseudomonas aeruginosa - analysis
Pyocins - isolation & purification
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Summary:The physicochemical properties of pyocin F1 were studied. Pyocin F1 consists of flexuous rod-like particles homogeneous in size. Each particle was composed of rod and fiber parts. The rod part was 105·5 ±9·5 nm long and 10·0± 1·4 nm wide, and showed regular striations amounting to 23 layers. The fiber part was composed of several filaments; the length of the longest filament was 43·0± 12·0 nm. The amino acid composition, the partial specific volume (0·720 ml/g), the sedimentation coefficient (s20,w0=35·1S) and the translational diffusion constant (0·94 ±0·01 × 10−7 cm2/s) were determined. The particle weight was calculated to be 3·23 × 106 daltons.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a132313