No effect of cAMP on protein synthesis in reticulocyte lysates

The results of a series of experiments are interpreted to indicate that protein synthesis in reticulocyte lysates is not affected by the reticulocyte cAMP-dependent protein kinase. The catalytic subunit of this enzyme was isolated to apparent homogeneity. Also, the protein inhibitor of this protein...

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Bibliographic Details
Published in:The Journal of biological chemistry 1979-05, Vol.254 (9), p.3145-3147
Main Authors: Grankowski, N, Kramer, G, Hardesty, B
Format: Article
Language:English
Subjects:
Animals
Blood Proteins - biosynthesis
Cyclic AMP - pharmacology
Edeine - pharmacology
Kinetics
Molecular Weight
Peptide Chain Initiation, Translational
Peptide Initiation Factors - metabolism
Phosphorylation
Protein Kinases - blood
Rabbits
Reticulocytes - enzymology
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Summary:The results of a series of experiments are interpreted to indicate that protein synthesis in reticulocyte lysates is not affected by the reticulocyte cAMP-dependent protein kinase. The catalytic subunit of this enzyme was isolated to apparent homogeneity. Also, the protein inhibitor of this protein kinase was isolated from muscle. Neither physiological concentrations of cAMP nor any of these protein components had a detectable effect on protein synthesis in reticulocyte lysates in the presence or absence of exogenous heme. Phosphorylation of the smallest subunit of eukaryotic initiation factor 2 or the 90,000 to 100,000-dalton peptide associated with eukaryotic initiation factor 2 kinase activity were not affected by the activity of the cAMP-dependent protein kinase under conditions in which exogenous heme has a pronounced effect on these reactions.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)50732-8