Protein Expression in the Livers and Urinary Bladders of Hamsters Exposed to Sodium Arsenite

Hamsters were exposed to sodium arsenite (173 mg As/L) in drinking water for 6 days and control hamsters were given tap water. Equal amounts of protein from the urinary bladder or liver extracts of control and arsenic‐treated hamsters were labeled with Cy3 and Cy5 dyes, respectively. The labeled pro...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2008-10, Vol.1140 (1), p.325-334
Main Authors: Chowdhury, Uttam K., Aposhian, H. Vasken
Format: Article
Language:English
Subjects:
Animals
Arsenic
arsenite
Arsenites - analysis
bladder
Cricetinae
differential in gel electrophoresis
Electrophoresis, Gel, Two-Dimensional
fluorescence
Gene Expression Regulation
hamster
liver
Liver - drug effects
Liver - metabolism
Male
Mass Spectrometry
Mesocricetus
protein expression
Proteome - analysis
Proteomics - methods
Sodium Compounds - analysis
Software
Urinary Bladder - drug effects
Urinary Bladder - metabolism
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Summary:Hamsters were exposed to sodium arsenite (173 mg As/L) in drinking water for 6 days and control hamsters were given tap water. Equal amounts of protein from the urinary bladder or liver extracts of control and arsenic‐treated hamsters were labeled with Cy3 and Cy5 dyes, respectively. The labeled proteins were mixed and separated in the two‐dimensional differential in gel electrophoresis (2D‐DIGE). After DIGE and analysis by the DeCyder software, several protein spots were found to be overexpressed and several were underexpressed. Analysis of the DIGE gel images detected 75 protein spots in the livers and 52 protein spots in the urinary bladders of hamsters that were expressed (±1.2‐fold). Of the detected protein spots, 34 spots were overexpressed (1.48 ± 0.05) and 41 spots were underexpressed (1.52 ± 0.06) in the liver. In the urinary bladder, 36 protein spots were overexpressed (1.52 ± 0.06) and 16 protein spots were underexpressed (1.39 ± 0.05). Three proteins (one was overexpressed and two were underexpressed) of each tissue (liver or bladder) were identified by mass spectrometry. DIGE in combination with mass spectrometry is a powerful tool that may be of help in understanding the molecular mechanisms of cancer progression due to inorganic arsenic.
ISSN:0077-8923
1749-6632
1930-6547
DOI:10.1196/annals.1454.019