Identification of TCP10L as primate-specific gene derived via segmental duplication and homodimerization of TCP10L through the leucine zipper motif

TCP10L, a transcriptional repression factor gene that was localized on human chromosome 21q22.11, was identified to be derived through segmental duplication since the divergence of primates and rodents. It was elucidated that TCP10L gene was a primate-specific gene in this study. Subsequently it was...

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Bibliographic Details
Published in:Molecular biology reports 2008-06, Vol.35 (2), p.171-178
Main Authors: Zhong, Zhaomin, Qiu, Jianping, Chen, Xinya, Wan, Bo, Ni, Jun, Yang, Yun, Bai, Meirong, Zhang, Haoxing, Yu, Long
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animals
Biomedical and Life Sciences
Cell Line
Cell Nucleus - metabolism
chromosome 21
Dimerization
Gene deletion
Gene Duplication
Gene silencing
Genes
Genome
Histology
Homodimerization
Humans
Immunofluorescence
Immunoprecipitation
leucine zipper
Leucine zipper proteins
Leucine Zippers
Life Sciences
Microtubule-Associated Proteins - chemistry
Microtubule-Associated Proteins - genetics
Molecular biology
Morphology
Nuclear Proteins - chemistry
Nuclear Proteins - genetics
Nuclei
Point mutation
Primate-specific
Primates
Primates - genetics
Protein Transport
Species Specificity
Studies
t-Complex Genome Region
TCP10
TCP10L
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Summary:TCP10L, a transcriptional repression factor gene that was localized on human chromosome 21q22.11, was identified to be derived through segmental duplication since the divergence of primates and rodents. It was elucidated that TCP10L gene was a primate-specific gene in this study. Subsequently it was demonstrated that the putative leucine zipper motif mediated the homodimerization of TCP10L. Using in vitro and in vivo methodologies, it was shown that either deletion or point mutation of the leucine zipper motif was sufficient to abolish TCP10L homodimerization. In Hela cells, both the exogenous wild type TCP10L and endogenous TCP10L were detected on nuclei with immunofluorescence assay. However, the leucine zipper motif mutants of TCP10L could also be detected on nuclei. The results suggested that the leucine zipper motif enabled TCP10L to homodimerize, but was not essential for the TCP10L nuclear localization.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-007-9067-5