Characterization and expression profiling of a novel cereal cyst nematode resistance gene analog in wheat

• Published in: Molecular biology (New York) 2008-12, Vol.42 (6), p.960-965
• Main Authors: Zhai, X. G, Zhao, T, Liu, Y. H, Long, H, Deng, G. B, Pan, Z. F, Yu, M. Q
• Format: Article
• Language: English
• Subjects: Amino acid sequence; Amino acids; Applied Molecular Biology; Biochemistry; Biomedical and Life Sciences; Cereals; Conserved sequence; Cysts; Disease resistance; Gene expression; Gene families; Human Genetics; Life Sciences; Molecular biology; Nematoda; Nucleotide sequence; Open reading frames; Plant resistance; Polymerase chain reaction; Roots; Triticum aestivum
• ISSN: 0026-8933; 1608-3245
• DOI: 10.1134/S0026893308060186

Based on the conserved regions of known resistance genes, an NBS-LRR-type CCN resistance gene analog was isolated from the CCN resistant E-10 near isogenic lines (NILs) of wheat, designated as CreZ (GenBank accession number: EU327996). It contained a complete ORF that was 2775 bp in length and encoded 924 amino acids. Sequence comparison indicated that it shared 92% nucleotide and 87% amino acid identity with those of the known CCN-resistance gene Cre3 and had similar characteristic conserved motifs to those in other established NBS-LRR disease resistance genes. The expression profiling of CreZ indicated that it was specifically expressed in the roots of resistant plants and real-time PCR analysis demonstrated that expression levels drastically increased when the plants were inoculated with cereal cyst nematodes. It could be inferred, then, that CreZ belongs to the NBS-LRR resistance gene family and is a candidate gene for potential resistance to the cereal cyst nematode.