Methods to improve the yield and quality of DNA from dried and processed figs

We describe here a molecular method that can be used to detect genome traits of a given horticultural item at each stage from the farm to the market. We developed a procedure to extract and amplify by PCR DNA obtained from complex matrixes, such as dried figs and fig jam. Few fragmented DNA molecule...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology progress 2005, Vol.21 (2), p.546-549
Main Authors: Di Bernardo, G, Galderisi, U, Cipollaro, M, Cascino, A
Format: Article
Language:English
Subjects:
Base Sequence
cultivars
DNA
DNA Primers
DNA, Plant - isolation & purification
dried fruit
Electrophoresis, Agar Gel
extraction
Ficus - genetics
fig jam
figs
food analysis
genes
Genomes
jams
Polymerase Chain Reaction
Q1
ribosomal RNA
traceability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We describe here a molecular method that can be used to detect genome traits of a given horticultural item at each stage from the farm to the market. We developed a procedure to extract and amplify by PCR DNA obtained from complex matrixes, such as dried figs and fig jam. Few fragmented DNA molecules can be recovered from food products. However, we were able to increase the yield of PCR reactions by successfully applying an enzymatic repair protocol to retrieved DNA.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp049710p