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Methods to improve the yield and quality of DNA from dried and processed figs
We describe here a molecular method that can be used to detect genome traits of a given horticultural item at each stage from the farm to the market. We developed a procedure to extract and amplify by PCR DNA obtained from complex matrixes, such as dried figs and fig jam. Few fragmented DNA molecule...
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| Published in: | Biotechnology progress 2005, Vol.21 (2), p.546-549 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c4550-364636df0847944a120e4ba733ce344721749d34daa4922e39c452b9bf4b48e43 |
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| cites | cdi_FETCH-LOGICAL-c4550-364636df0847944a120e4ba733ce344721749d34daa4922e39c452b9bf4b48e43 |
| container_end_page | 549 |
| container_issue | 2 |
| container_start_page | 546 |
| container_title | Biotechnology progress |
| container_volume | 21 |
| creator | Di Bernardo, G Galderisi, U Cipollaro, M Cascino, A |
| description | We describe here a molecular method that can be used to detect genome traits of a given horticultural item at each stage from the farm to the market. We developed a procedure to extract and amplify by PCR DNA obtained from complex matrixes, such as dried figs and fig jam. Few fragmented DNA molecules can be recovered from food products. However, we were able to increase the yield of PCR reactions by successfully applying an enzymatic repair protocol to retrieved DNA. |
| doi_str_mv | 10.1021/bp049710p |
| format | article |
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| fulltext | fulltext |
| identifier | ISSN: 8756-7938 |
| ispartof | Biotechnology progress, 2005, Vol.21 (2), p.546-549 |
| issn | 8756-7938 1520-6033 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_744584846 |
| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Base Sequence cultivars DNA DNA Primers DNA, Plant - isolation & purification dried fruit Electrophoresis, Agar Gel extraction Ficus - genetics fig jam figs food analysis genes Genomes jams Polymerase Chain Reaction Q1 ribosomal RNA traceability |
| title | Methods to improve the yield and quality of DNA from dried and processed figs |
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