Determination of Binding Parameters and Mode of Ferrocenyl Chalcone–DNA Interaction

• Published in: Bulletin of the Chemical Society of Japan 2009-01, Vol.82 (4), p.453-457
• Main Authors: Shah, Afzal, Qureshi, Rumana, Khan, Asad Muhammad, Ansari, Farzana Latif, Ahmad, Safeer
• Format: Article
• Language: English
• ISSN: 0009-2673; 1348-0634
• DOI: 10.1246/bcsj.82.453

This paper reports that how the variation in peak current, absorbance, and viscosity of ferrocenyl chalcone (FC), chemically named as 1-ferrocenyl-3-(4-nitrophenyl)-2-propen-1-one, in 10% aqueous DMF upon addition of DNA can be used to probe the mode of interaction and binding parameters. Binding constant (K = 5.17 (±0.25) × 103 M−1), binding site size (s = 1.08 ± 0.05 bp) and diffusion coefficient of the free (Df = 5.22 × 10−7 cm2 s−1) and DNA bound drug (Db = 4.39 × 10−8 cm2 s−1) were determined from voltammetric data. The binding constant (K = 4.91 (±0.20) × 103 M−1) was also obtained from UV–vis absorption titration. Gibbs energy change (ΔG = −RT ln K) of −21.18 kJ mol−1 at 25 °C indicated the spontaneity of the binding interaction. The experimental results revealed intercalation of FC into DNA as the dominant mode of interaction. Furthermore, the radii of the free and DNA-bound drug were determined from viscosity measurements.