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Morphological and Molecular Characterization of the Causal Agent of Downy Mildew on Quinoa (Chenopodium quinoa)
Downy mildew is an economically important and widespread disease in quinoa ( Chenopodium quinoa ) growing areas. Although in many studies Peronospora farinosa is most commonly regarded as the causal agent of the disease, identification and classification of the pathogen remain still uncertain due to...
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| Published in: | Mycopathologia (1975) 2010-05, Vol.169 (5), p.403-412 |
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| Main Authors: | , , , , , |
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| container_end_page | 412 |
| container_issue | 5 |
| container_start_page | 403 |
| container_title | Mycopathologia (1975) |
| container_volume | 169 |
| creator | Choi, Young-Joon Danielsen, Solveig Lübeck, Mette Hong, Seung-Beom Delhey, Rolf Shin, Hyeon-Dong |
| description | Downy mildew is an economically important and widespread disease in quinoa (
Chenopodium quinoa
) growing areas. Although in many studies
Peronospora farinosa
is most commonly regarded as the causal agent of the disease, identification and classification of the pathogen remain still uncertain due to its taxonomic confusion. Thirty-six
Peronospora
isolates from quinoa with different geographic origins including Argentina, Bolivia, Denmark, Ecuador, and Peru were morphologically and molecularly compared with
Peronospora
species from other
Chenopodium
species. The morphology of three herbarium specimens was similar to that of
P.
variabilis
, which originated from
C. album
, characterized by flexuous to curved ultimate branchlets and pedicellated conidia. Phylogenetic analysis based on ITS rDNA sequences also placed the quinoa pathogen within the same clade as
P. variabilis
. Within the ITS rDNA sequences of the quinoa pathogens, two base substitutions were found, which separated the majority of the Danish isolates from isolates from South America, but no sequence difference was found among the isolates from different cultivars of quinoa. The present results indicate that the pathogen responsible for the quinoa downy mildew is identical to
Peronospora variabilis
and that it should not be lumped with
P. farinosa
as claimed previously by most studies. |
| doi_str_mv | 10.1007/s11046-010-9272-y |
| format | article |
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Chenopodium quinoa
) growing areas. Although in many studies
Peronospora farinosa
is most commonly regarded as the causal agent of the disease, identification and classification of the pathogen remain still uncertain due to its taxonomic confusion. Thirty-six
Peronospora
isolates from quinoa with different geographic origins including Argentina, Bolivia, Denmark, Ecuador, and Peru were morphologically and molecularly compared with
Peronospora
species from other
Chenopodium
species. The morphology of three herbarium specimens was similar to that of
P.
variabilis
, which originated from
C. album
, characterized by flexuous to curved ultimate branchlets and pedicellated conidia. Phylogenetic analysis based on ITS rDNA sequences also placed the quinoa pathogen within the same clade as
P. variabilis
. Within the ITS rDNA sequences of the quinoa pathogens, two base substitutions were found, which separated the majority of the Danish isolates from isolates from South America, but no sequence difference was found among the isolates from different cultivars of quinoa. The present results indicate that the pathogen responsible for the quinoa downy mildew is identical to
Peronospora variabilis
and that it should not be lumped with
P. farinosa
as claimed previously by most studies.</description><identifier>ISSN: 0301-486X</identifier><identifier>EISSN: 1573-0832</identifier><identifier>DOI: 10.1007/s11046-010-9272-y</identifier><identifier>PMID: 20101524</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Chemical properties ; Chenopodium ; Chenopodium quinoa ; Chenopodium quinoa - microbiology ; Cladistic analysis ; Crop diseases ; Denmark ; DNA, Algal - genetics ; DNA, Ribosomal Spacer - genetics ; Eukaryotic Microbiology ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbiology ; Morphology ; Pathogens ; Peronospora ; Peronospora - classification ; Peronospora - cytology ; Peronospora - genetics ; Phylogenetics ; Phylogeny ; Plant Diseases - microbiology ; Plant Sciences ; Quinoa ; South America ; Taxonomy</subject><ispartof>Mycopathologia (1975), 2010-05, Vol.169 (5), p.403-412</ispartof><rights>Springer Science+Business Media B.V. 2010</rights><rights>COPYRIGHT 2010 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-84e0e881982a878542710e885daafcf42ad230a8d266f98954d466001921d573</citedby><cites>FETCH-LOGICAL-c475t-84e0e881982a878542710e885daafcf42ad230a8d266f98954d466001921d573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20101524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choi, Young-Joon</creatorcontrib><creatorcontrib>Danielsen, Solveig</creatorcontrib><creatorcontrib>Lübeck, Mette</creatorcontrib><creatorcontrib>Hong, Seung-Beom</creatorcontrib><creatorcontrib>Delhey, Rolf</creatorcontrib><creatorcontrib>Shin, Hyeon-Dong</creatorcontrib><title>Morphological and Molecular Characterization of the Causal Agent of Downy Mildew on Quinoa (Chenopodium quinoa)</title><title>Mycopathologia (1975)</title><addtitle>Mycopathologia</addtitle><addtitle>Mycopathologia</addtitle><description>Downy mildew is an economically important and widespread disease in quinoa (
Chenopodium quinoa
) growing areas. Although in many studies
Peronospora farinosa
is most commonly regarded as the causal agent of the disease, identification and classification of the pathogen remain still uncertain due to its taxonomic confusion. Thirty-six
Peronospora
isolates from quinoa with different geographic origins including Argentina, Bolivia, Denmark, Ecuador, and Peru were morphologically and molecularly compared with
Peronospora
species from other
Chenopodium
species. The morphology of three herbarium specimens was similar to that of
P.
variabilis
, which originated from
C. album
, characterized by flexuous to curved ultimate branchlets and pedicellated conidia. Phylogenetic analysis based on ITS rDNA sequences also placed the quinoa pathogen within the same clade as
P. variabilis
. Within the ITS rDNA sequences of the quinoa pathogens, two base substitutions were found, which separated the majority of the Danish isolates from isolates from South America, but no sequence difference was found among the isolates from different cultivars of quinoa. The present results indicate that the pathogen responsible for the quinoa downy mildew is identical to
Peronospora variabilis
and that it should not be lumped with
P. farinosa
as claimed previously by most studies.</description><subject>Biomedical and Life Sciences</subject><subject>Chemical properties</subject><subject>Chenopodium</subject><subject>Chenopodium quinoa</subject><subject>Chenopodium quinoa - microbiology</subject><subject>Cladistic analysis</subject><subject>Crop diseases</subject><subject>Denmark</subject><subject>DNA, Algal - genetics</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Eukaryotic Microbiology</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Morphology</subject><subject>Pathogens</subject><subject>Peronospora</subject><subject>Peronospora - classification</subject><subject>Peronospora - cytology</subject><subject>Peronospora - genetics</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Sciences</subject><subject>Quinoa</subject><subject>South America</subject><subject>Taxonomy</subject><issn>0301-486X</issn><issn>1573-0832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkk1v1DAQhi0EotvCD-CCLC60hxR_JXaOq_BVqSsE9MDNMvEk6yprb-1EZfn1OKSAioSQD7ZePzPjGb8IPaPknBIiXyVKiagKQklRM8mKwwO0oqXkBVGcPUQrwgkthKq-HKHjlK5JBgmVj9ERyydaMrFCYRPifhuG0LvWDNh4izdhgHYaTMTN1kTTjhDddzO64HHo8LgF3JgpZXjdgx9n7XW49Qe8cYOFW5yxj5PzweDTZgs-7IN10w7f_NTOnqBHnRkSPL3bT9DV2zdXzfvi8sO7i2Z9WbRClmOhBBBQitaKGSVVKZiks1BaY7q2E8xYxolRllVVV6u6FFZUVe6uZtTmCZygl0vafQw3E6RR71xqYRiMhzAlLYUoaypl-X-Sc6WEqFUmX_xFXocp-tyFZoxSLjghGTpfoN4MoJ3vwphHmJeFnWuDh85lfc3LnLSmfK5_di8gMyN8G_s84qQvPn-6z9KFbWNIKUKn99HtTDxoSvTsCL04Qufv1bMj9CHHPL979fR1B_Z3xC8LZIAtQMpXvof4p61_Z_0BA6C-ag</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Choi, Young-Joon</creator><creator>Danielsen, Solveig</creator><creator>Lübeck, Mette</creator><creator>Hong, Seung-Beom</creator><creator>Delhey, Rolf</creator><creator>Shin, Hyeon-Dong</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20100501</creationdate><title>Morphological and Molecular Characterization of the Causal Agent of Downy Mildew on Quinoa (Chenopodium quinoa)</title><author>Choi, Young-Joon ; Danielsen, Solveig ; Lübeck, Mette ; Hong, Seung-Beom ; Delhey, Rolf ; Shin, Hyeon-Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-84e0e881982a878542710e885daafcf42ad230a8d266f98954d466001921d573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biomedical and Life Sciences</topic><topic>Chemical properties</topic><topic>Chenopodium</topic><topic>Chenopodium quinoa</topic><topic>Chenopodium quinoa - microbiology</topic><topic>Cladistic analysis</topic><topic>Crop diseases</topic><topic>Denmark</topic><topic>DNA, Algal - genetics</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Eukaryotic Microbiology</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Morphology</topic><topic>Pathogens</topic><topic>Peronospora</topic><topic>Peronospora - classification</topic><topic>Peronospora - cytology</topic><topic>Peronospora - genetics</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Sciences</topic><topic>Quinoa</topic><topic>South America</topic><topic>Taxonomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Young-Joon</creatorcontrib><creatorcontrib>Danielsen, Solveig</creatorcontrib><creatorcontrib>Lübeck, Mette</creatorcontrib><creatorcontrib>Hong, Seung-Beom</creatorcontrib><creatorcontrib>Delhey, Rolf</creatorcontrib><creatorcontrib>Shin, Hyeon-Dong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Science (Gale in Context)</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Databases</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Mycopathologia (1975)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Young-Joon</au><au>Danielsen, Solveig</au><au>Lübeck, Mette</au><au>Hong, Seung-Beom</au><au>Delhey, Rolf</au><au>Shin, Hyeon-Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological and Molecular Characterization of the Causal Agent of Downy Mildew on Quinoa (Chenopodium quinoa)</atitle><jtitle>Mycopathologia (1975)</jtitle><stitle>Mycopathologia</stitle><addtitle>Mycopathologia</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>169</volume><issue>5</issue><spage>403</spage><epage>412</epage><pages>403-412</pages><issn>0301-486X</issn><eissn>1573-0832</eissn><abstract>Downy mildew is an economically important and widespread disease in quinoa (
Chenopodium quinoa
) growing areas. Although in many studies
Peronospora farinosa
is most commonly regarded as the causal agent of the disease, identification and classification of the pathogen remain still uncertain due to its taxonomic confusion. Thirty-six
Peronospora
isolates from quinoa with different geographic origins including Argentina, Bolivia, Denmark, Ecuador, and Peru were morphologically and molecularly compared with
Peronospora
species from other
Chenopodium
species. The morphology of three herbarium specimens was similar to that of
P.
variabilis
, which originated from
C. album
, characterized by flexuous to curved ultimate branchlets and pedicellated conidia. Phylogenetic analysis based on ITS rDNA sequences also placed the quinoa pathogen within the same clade as
P. variabilis
. Within the ITS rDNA sequences of the quinoa pathogens, two base substitutions were found, which separated the majority of the Danish isolates from isolates from South America, but no sequence difference was found among the isolates from different cultivars of quinoa. The present results indicate that the pathogen responsible for the quinoa downy mildew is identical to
Peronospora variabilis
and that it should not be lumped with
P. farinosa
as claimed previously by most studies.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>20101524</pmid><doi>10.1007/s11046-010-9272-y</doi><tpages>10</tpages></addata></record> |
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| subjects | Biomedical and Life Sciences Chemical properties Chenopodium Chenopodium quinoa Chenopodium quinoa - microbiology Cladistic analysis Crop diseases Denmark DNA, Algal - genetics DNA, Ribosomal Spacer - genetics Eukaryotic Microbiology Life Sciences Medical Microbiology Microbial Ecology Microbiology Morphology Pathogens Peronospora Peronospora - classification Peronospora - cytology Peronospora - genetics Phylogenetics Phylogeny Plant Diseases - microbiology Plant Sciences Quinoa South America Taxonomy |
| title | Morphological and Molecular Characterization of the Causal Agent of Downy Mildew on Quinoa (Chenopodium quinoa) |
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