Molecular cloning, characterization and function analysis of the gene encoding HMG-CoA reductase from Euphorbia Pekinensis Rupr

• Published in: Molecular biology reports 2010-03, Vol.37 (3), p.1559-1567
• Main Authors: Cao, Xiaoying, Zong, Zhimin, Ju, Xiuyun, Sun, Yong, Dai, Chuanchao, Liu, Qun, Jiang, Jihong
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Base Sequence; Biomedical and Life Sciences; Blotting, Southern; Carotenoids - biosynthesis; Cloning; Cloning, Molecular; Computational Biology; DNA Primers - genetics; DNA, Complementary - genetics; Enzymes; Escherichia coli; Euphorbia; Euphorbia - enzymology; Gene Expression Profiling; Genes; Histology; Hydroxymethylglutaryl CoA Reductases - genetics; Hydroxymethylglutaryl CoA Reductases - metabolism; Life Sciences; Models, Molecular; Molecular biology; Molecular Sequence Data; Morphology; Pharmacology; Plant biology; Plant Leaves - chemistry; Plant Roots - metabolism; Sequence Analysis, DNA; Sequence Homology
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-009-9558-7

A new full-length cDNA encoding 3-hydroxy-3-methylglutoryl-Coenzyme A reductase (HMGR; EC1.1.1.34), which catalyzes the first committed step of isoprenoids biosynthesis in MVA pathway, was isolated from young leaves of Euphorbia Pekinensis Rupr. by rapid amplification of cDNA ends (RACE) for the first time. The full-length cDNA of HMGR (designated as EpHMGR, GenBank Accession NO. EF062569) was 2,200 bp containing a 1,752 bp ORF encoding 583 amino acids. Bioinformatic analyzes revealed that the deduced EpHMGR had extensive homology with other plant HMGRs and contained two transmembrane domains and a catalytic domain. The predicted 3-D model of EpHMGR had a typical spatial structure of HMGRs. Southern blot analysis indicated that at most two copies of EpHMGR gene existed in E. Pekinensis genome. Tissue expression analysis revealed that EpHMGR expressed strongly in roots, weakly in stems and leaves. The functional colour complementation assay indicated that EpHMGR could accelerate the biosynthesis of carotenoids in the Escherichia coli transformant, demonstrating that EpHMGR plays an influential role in isoprenoid biosynthesis.