Methylation of the MGMT Gene is Frequently Detected in Advanced Gastric Carcinoma

Background: Recently, Herfarth et al. reported that a subset of colorectal tumors was characterized by a specific methylation pattern in the MGMT promoter associated with reduced MGMT expression. Materials and Methods: Methylation status of the MGMT gene was examined in primary carcinomas and the co...

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Bibliographic Details
Published in:Anticancer research 2009-12, Vol.29 (12), p.5053-5055
Main Authors: Hibi, Kenji, Sakata, Makiko, Yokomizo, Kazuaki, Kitamura, Yo-Hei, Sakuraba, Kazuma, Shirahata, Atsushi, Goto, Tetsuhiro, Mizukami, Hiroki, Saito, Mitsuo, Ishibashi, Kazuyoshi, Kigawa, Gaku, Nemoto, Hiroshi, Sanada, Yutaka
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
DNA Methylation
DNA Modification Methylases - genetics
DNA Repair Enzymes - genetics
Female
Gene Expression Regulation, Neoplastic
Humans
Lymphatic Metastasis
Male
Middle Aged
Neoplasm Staging
Polymerase Chain Reaction
Prognosis
Promoter Regions, Genetic - genetics
Stomach - metabolism
Stomach - pathology
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Tumor Suppressor Proteins - genetics
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Summary:Background: Recently, Herfarth et al. reported that a subset of colorectal tumors was characterized by a specific methylation pattern in the MGMT promoter associated with reduced MGMT expression. Materials and Methods: Methylation status of the MGMT gene was examined in primary carcinomas and the corresponding normal tissues derived from 38 patients with gastric cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated. Results: Aberrant methylation of the MGMT gene was detected in 4 out of the 38 (11%) primary gastric carcinomas, suggesting that the methylation of MGMT is observed in gastric carcinomas as well as colorectal ones. The clinicopathological data were correlated with the methylation results. A significant difference was observed in the extent of tumor (p=0.0470), lymph node metastasis (p=0.0470), and TNM stage (p=0.0377) ( Table I ). Moreover, a trend was shown toward large maximal tumor size in methylated tumors (p=0.134). Conclusion: MGMT was more frequently methylated in advanced gastric carcinomas.
ISSN:0250-7005
1791-7530