Enhanced Chondrogenic Responses of Human Articular Chondrocytes Onto Silk Fibroin/Wool Keratose Scaffolds Treated With Microwave-Induced Argon Plasma

Silk fibroin (SF) is a natural, degradable, fibrous protein that is biocompatible, is easily processed, and possesses unique mechanical properties. Another natural material, wool keratose (WK), is a soluble derivative of wool keratin, containing amino acid sequences that induce cell adhesion. Here,...

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Bibliographic Details
Published in:Artificial organs 2010-05, Vol.34 (5), p.384-392
Main Authors: Cheon, Young Woo, Lee, Won Jai, Baek, Hyun Sook, Lee, Young Dae, Park, Jong-Chul, Park, Young Hwan, Ki, Chang Seok, Chung, Kie-Hyung, Rah, Dong Kyun
Format: Article
Language:English
Subjects:
Animals
Argon - chemistry
Bombyx - chemistry
Cartilage tissue engineering
Cartilage, Articular - cytology
Cell Adhesion
Cell Proliferation
Cells, Cultured
Chondrocytes
Chondrocytes - cytology
Chondrocytes - metabolism
Fibroins - chemistry
Fibroins - isolation & purification
Glycosaminoglycans - metabolism
Humans
Keratins - chemistry
Microwave-induced argon plasma
Microwaves
Nanofibrous silk fibroin
Porosity
Scaffold
Surface Properties
Tissue Scaffolds
Water - chemistry
Wool - chemistry
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Summary:Silk fibroin (SF) is a natural, degradable, fibrous protein that is biocompatible, is easily processed, and possesses unique mechanical properties. Another natural material, wool keratose (WK), is a soluble derivative of wool keratin, containing amino acid sequences that induce cell adhesion. Here, we blended SF and WK to improve the poor electrospinability of WK and increase the adhesiveness of SF. We hypothesized that microwave‐induced argon plasma treatment would improve chondrogenic cell growth and cartilage‐specific extracellular matrix formation on a three‐dimensional SF/WK scaffold. After argon plasma treatment, static water contact angle measurement revealed increased hydrophilicity of the SF/WK scaffold, and scanning electron microscopy showed that treated SF/WK scaffolds had deeper and more cylindrical pores than nontreated scaffolds. Attachment and proliferation of neonatal human knee articular chondrocytes on treated SF/WK scaffolds increased significantly, followed by increased glycosaminoglycan synthesis. Our results suggest that microwave‐induced, plasma‐treated SF/WK scaffolds have potential in cartilage tissue engineering.
ISSN:0160-564X
1525-1594
DOI:10.1111/j.1525-1594.2009.00871.x