Polymeric piezoelectric actuator substrate for osteoblast mechanical stimulation

Abstract Bone mass distribution and structure are dependent on mechanical stress and adaptive response at cellular and tissue levels. Mechanical stimulation of bone induces new bone formation in vivo and increases the metabolic activity and gene expression of osteoblasts in culture. A wide variety o...

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Bibliographic Details
Published in:Journal of biomechanics 2010-04, Vol.43 (6), p.1061-1066
Main Authors: Frias, C, Reis, J, Capela e Silva, F, Potes, J, Simões, J, Marques, A.T
Format: Article
Language:English
Subjects:
3T3 Cells
Actuator
Animals
Apoptosis
Biological and medical sciences
Biomechanical Phenomena
Biomechanics. Biorheology
Bone Remodeling - physiology
Cell adhesion & migration
Cell culture
Cell Survival
Coated Materials, Biocompatible
Electrodes
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Interferometry
Mechanical stimulation
Medical sciences
Membranes, Artificial
Mice
Models, Biological
Nitric oxide
Nitric Oxide - metabolism
Osteoblast cells
Osteoblasts - cytology
Osteoblasts - physiology
Physical Medicine and Rehabilitation
Polymeric piezoelectric
Polyvinyls
Proteins
Stress, Mechanical
Studies
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
Tissues, organs and organisms biophysics
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Summary:Abstract Bone mass distribution and structure are dependent on mechanical stress and adaptive response at cellular and tissue levels. Mechanical stimulation of bone induces new bone formation in vivo and increases the metabolic activity and gene expression of osteoblasts in culture. A wide variety of devices have been tested for mechanical stimulation of cells and tissues in vitro. The aim of this work was to experimentally validate the possibility to use piezoelectric materials as a mean of mechanical stimulation of bone cells, by converse piezoelectric effect. To estimate the magnitude and the distribution of strain, finite numerical models were applied and the results were complemented with the optical tests (Electronic Speckle Pattern Interferometric Process). In this work, osteoblasts were grown on the surface of a piezoelectric material, both in static and dynamic conditions at low frequencies, and total protein, cell viability and nitric oxide measurement comparisons are presented.
ISSN:0021-9290
1873-2380
DOI:10.1016/j.jbiomech.2009.12.010