Analysis of genetic and biological characters of Japanese potato strains of Ralstonia solanacearum

We assessed the geographic distribution, biovar, phylotype, DNA fingerprints (rep-PCR), and/or endoglucanase sequence of potato bacterial wilt pathogen, Ralstonia solanacearum (Rs), in Japan. Rs has been isolated from potato fields in southwestern, warm, temperate regions. Of the 188 isolates, 74 be...

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Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2010-06, Vol.76 (3), p.196-207
Main Authors: Horita, Mitsuo, Suga, Yasuhiro, Ooshiro, Atsushi, Tsuchiya, Kenichi
Format: Article
Language:English
Subjects:
Agriculture
Analysis
Bacterial and Phytoplasma Diseases
Biology
Biomedical and Life Sciences
Biovar
Deoxyribonucleic acid
DNA
Endoglucanase
Genetic diversity
Genetics
Geographical distribution
Life Sciences
Microbiology
Phylotype
Plant Pathology
Potatoes
Ralstonia solanacearum
Solanum tuberosum
Vegetables
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Summary:We assessed the geographic distribution, biovar, phylotype, DNA fingerprints (rep-PCR), and/or endoglucanase sequence of potato bacterial wilt pathogen, Ralstonia solanacearum (Rs), in Japan. Rs has been isolated from potato fields in southwestern, warm, temperate regions. Of the 188 isolates, 74 belonged to biovar N2 (39%), 44 to biovar 3 (24%), and 70 to biovar 4 (37%). Biovars N2 and 4 strains were widely distributed, from northern (Hokkaido) to southern (Okinawa) Japan. Based on the results of multiplex-PCR analysis, every potato strains belonged to either phylotype I or IV. Phylotype I comprised both biovars 3 and 4 strains. On the other hand, phylotype IV included biovar N2 strains. None of the strains belonged to phylotype II or III or biovar 1 or 2. Phylogenetic analysis based on DNA fingerprints and endoglucanase gene sequences clarified the genetic diversity of the Japanese potato strains and the close genetic relationship between the Japanese strains and the Asian strains in phylotypes I and IV.
ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-010-0229-2