Novel role of Acinetobacter baumannii RND efflux transporters in mediating decreased susceptibility to biocides

Objectives Biocides and dyes are commonly employed in hospital and laboratory settings. We investigated the biocide susceptibilities of a rapidly emerging pathogen, Acinetobacter baumannii, and the underlying molecular mechanisms, with a primary focus on resistance–nodulation–cell division (RND) eff...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2010-02, Vol.65 (2), p.228-232
Main Authors: Rajamohan, Govindan, Srinivasan, Vijaya Bharathi, Gebreyes, Wondwossen A.
Format: Article
Language:English
Subjects:
Acinetobacter baumannii
Acinetobacter baumannii - drug effects
Acinetobacter baumannii - genetics
Antibiotics. Antiinfectious agents. Antiparasitic agents
antimicrobial resistance
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological and medical sciences
Cell division
Chemical compounds
Coloring Agents - pharmacology
Disinfectants - pharmacology
DNA, Bacterial - genetics
Drug Resistance, Bacterial
Dyes
Gene expression
Gene Expression Profiling
Gene Knockout Techniques
Humans
Medical sciences
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
Microbial Sensitivity Tests
multidrug efflux
Mutation
Pharmacology. Drug treatments
Polymerase Chain Reaction - methods
Reverse Transcriptase Polymerase Chain Reaction
RND efflux pump
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Summary:Objectives Biocides and dyes are commonly employed in hospital and laboratory settings. We investigated the biocide susceptibilities of a rapidly emerging pathogen, Acinetobacter baumannii, and the underlying molecular mechanisms, with a primary focus on resistance–nodulation–cell division (RND) efflux systems. Methods Biocide susceptibilities, efflux and in vitro inactivation profiles were monitored in the presence/absence of efflux pump inhibitors. The RND transporters encoded by adeB and adeJ were detected by PCR; null mutants were constructed in the native host. Expression of adeB and adeJ in clinical isolates was assayed by semi-quantitative RT–PCR. Results Susceptibility testing and phenotypic assays demonstrated the role of active efflux in mediating decreased susceptibility to biocides. Inactivation of either the adeB or adeJ transporter gene led to increased susceptibility to biocides. RT–PCR analysis exhibited increased adeB and adeJ expression in clinical isolates. Conclusions This is the first study demonstrating the role of efflux pumps in mediating decreased susceptibility to disinfectants and other chemical substrates in A. baumannii.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/dkp427